A Quninolylthiazole Derivatives as an ICT-Based Fluorescent Probe of Hg(II) and its Application in Ratiometric Imaging in Live HeLa Cells

A Quninolylthiazole Derivatives as an ICT-Based Fluorescent Probe of Hg(II) and its Application... As a structural analogue of pyridylthiazole, 2-(2-benzothiazoyl)-phenylethynylquinoline (QBT) was designed as a fluorescent probe for Hg(II) based on an intramolecular charge transfer (ICT) mechanism. The compound was synthesized in three steps starting from 6-bromo-2-methylquinoline, with moderate yield. Corresponding studies on the optical properties of QBT indicate that changes in the fluorescence ratio of QBT in response to Hg(II) could be quantified based on dual-emission changes. More specifically, the emission spectrum of QBT before and after interactions with Hg(II) exhibited a remarkable red shift of about 120 nm, which is rarely reported in ICT-based fluorescent sensors. Finally, QBT was applied in the two-channel imaging of Hg(II) in live HeLa cells. . . . . . Keywords Fluorescence Pyridylthiazole Ratiometric sensor Mercury ion ICT Imaging Introduction sensitivity, fluorometric methods are advantageous for the analysis of biological and environmental samples [6]. Many Mercury is one of the most toxic metal species, due to its fluorescent sensors for Hg(II) have been developed based on bioaccumulation and persistence in the environment [1–3]. It various organic fluorophores [7]. is known to adversely affect the neurological and endocrine Compared with the Boff-on^ and Bon-off^ single-emission systems, and thus lead to various malignant diseases, such as types http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Fluorescence Springer Journals

A Quninolylthiazole Derivatives as an ICT-Based Fluorescent Probe of Hg(II) and its Application in Ratiometric Imaging in Live HeLa Cells

Loading next page...
 
/lp/springer_journal/a-quninolylthiazole-derivatives-as-an-ict-based-fluorescent-probe-of-KMsOJuku3E
Publisher
Springer US
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Biomedicine; Biomedicine, general; Biological and Medical Physics, Biophysics; Biotechnology; Biochemistry, general; Analytical Chemistry
ISSN
1053-0509
eISSN
1573-4994
D.O.I.
10.1007/s10895-018-2241-4
Publisher site
See Article on Publisher Site

Abstract

As a structural analogue of pyridylthiazole, 2-(2-benzothiazoyl)-phenylethynylquinoline (QBT) was designed as a fluorescent probe for Hg(II) based on an intramolecular charge transfer (ICT) mechanism. The compound was synthesized in three steps starting from 6-bromo-2-methylquinoline, with moderate yield. Corresponding studies on the optical properties of QBT indicate that changes in the fluorescence ratio of QBT in response to Hg(II) could be quantified based on dual-emission changes. More specifically, the emission spectrum of QBT before and after interactions with Hg(II) exhibited a remarkable red shift of about 120 nm, which is rarely reported in ICT-based fluorescent sensors. Finally, QBT was applied in the two-channel imaging of Hg(II) in live HeLa cells. . . . . . Keywords Fluorescence Pyridylthiazole Ratiometric sensor Mercury ion ICT Imaging Introduction sensitivity, fluorometric methods are advantageous for the analysis of biological and environmental samples [6]. Many Mercury is one of the most toxic metal species, due to its fluorescent sensors for Hg(II) have been developed based on bioaccumulation and persistence in the environment [1–3]. It various organic fluorophores [7]. is known to adversely affect the neurological and endocrine Compared with the Boff-on^ and Bon-off^ single-emission systems, and thus lead to various malignant diseases, such as types

Journal

Journal of FluorescenceSpringer Journals

Published: May 28, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off