A possible mechanism for low affinity of silkworm Na+/K+-ATPase for K+

A possible mechanism for low affinity of silkworm Na+/K+-ATPase for K+ The affinity for K+ of silkworm nerve Na+/K+-ATPase is markedly lower than that of mammalian Na+/K+-ATPase (Homareda 2010). In order to obtain clues on the molecular basis of the difference in K+ affinities, we cloned cDNAs of silkworm (Bombyx mori) nerve Na+/K+-ATPase α and β subunits, and analyzed the deduced amino acid sequences. The molecular masses of the α and β subunits were presumed to be 111.5 kDa with ten transmembrane segments and 37.7 kDa with a single transmembrane segment, respectively. The α subunit showed 75% identity and 93% homology with the pig Na+/K+-ATPase α1 subunit. On the other hand, the amino acid identity of the β subunit with mammalian counterparts was as low as 30%. Cloned α and β cDNAs were co-expressed in cultured silkworm ovary-derived cells, BM-N cells, which lack endogenous Na+/K+-ATPase. Na+/K+-ATPase expressed in the cultured cells showed a low affinity for K+ and a high affinity for Na+, characteristic of the silkworm nerve Na+/K+-ATPase. These results suggest that the β subunit is responsible for the affinity for K+ of Na+/K+-ATPase. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Bioenergetics and Biomembranes Springer Journals

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Springer US
Copyright © 2017 by Springer Science+Business Media, LLC
Chemistry; Bioorganic Chemistry; Biochemistry, general; Animal Anatomy / Morphology / Histology; Animal Biochemistry; Organic Chemistry
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