A porcine circovirus-2 mutant isolated in Brazil contains low-frequency substitutions in regions of immunoprotective epitopes in the capsid protein

A porcine circovirus-2 mutant isolated in Brazil contains low-frequency substitutions in regions... Porcine circovirus-2 (PCV2) is the etiologic agent of several diseases in pigs, including multi-systemic wasting syndrome (PMWS). In this work, a new mutant PCV2b was isolated from PMWS-affected pigs on a Brazilian farm. Its genome showed high sequence similarity (>99 % identity) to those from a group of emerging mutants isolated from cases of PMWS outbreaks in vaccinated pigs in China, the USA and South Korea. Here, we show that these isolates share a combination of low-frequency substitutions (single amino acid polymorphisms with a frequency of ≤25 %) in the viral capsid protein, mainly in regions of immunoprotective epitopes, and an additional lysine residue at position 234. These isolates were phylogenetically grouped in the PCV2b clade, reinforcing the idea of the emergence of a new group of mutants PCV2b associated with outbreaks worldwide. The identification of these polymorphisms in the viral capsid highlights the importance of considering these isolates for the development of more-effective vaccines. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

A porcine circovirus-2 mutant isolated in Brazil contains low-frequency substitutions in regions of immunoprotective epitopes in the capsid protein

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Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2567-z
Publisher site
See Article on Publisher Site

Abstract

Porcine circovirus-2 (PCV2) is the etiologic agent of several diseases in pigs, including multi-systemic wasting syndrome (PMWS). In this work, a new mutant PCV2b was isolated from PMWS-affected pigs on a Brazilian farm. Its genome showed high sequence similarity (>99 % identity) to those from a group of emerging mutants isolated from cases of PMWS outbreaks in vaccinated pigs in China, the USA and South Korea. Here, we show that these isolates share a combination of low-frequency substitutions (single amino acid polymorphisms with a frequency of ≤25 %) in the viral capsid protein, mainly in regions of immunoprotective epitopes, and an additional lysine residue at position 234. These isolates were phylogenetically grouped in the PCV2b clade, reinforcing the idea of the emergence of a new group of mutants PCV2b associated with outbreaks worldwide. The identification of these polymorphisms in the viral capsid highlights the importance of considering these isolates for the development of more-effective vaccines.

Journal

Archives of VirologySpringer Journals

Published: Nov 1, 2015

References

  • Fine mapping of antigenic epitopes on capsid proteins of porcine circovirus, and antigenic phenotype of porcine circovirus type 2
    Shang, S-B; Jin, Y-L; Jiang, X; Zhou, J-Y; Zhang, X; Xing, G; He, JL; Yan, Y
  • Amino acid mutations in the capsid protein produce novel porcine circovirus type 2 neutralizing epitopes
    Liu, J; Huang, L; Wei, Y; Tang, Q; Liu, D; Wang, Y; Li, S; Guo, L; Wu, H; Liu, C
  • Effect of sow and piglet porcine circovirus type 2 (PCV2) vaccination on piglet mortality, viraemia, antibody titre and production parameters
    Fraile, L; Sibila, M; Nofrarías, M; López-Jimenez, R; Huerta, E; Llorens, A; López-Soria, S; Pérez, D; Segalés, J
  • One dose of a porcine circovirus 2 subunit vaccine induces humoral and cell-mediated immunity and protects against porcine circovirus-associated disease under field conditions
    Martelli, P; Ferrari, L; Morganti, M; Angelis, E; Bonilauri, P; Guazzetti, S; Caleffi, A; Borghetti, P
  • Genetic and antigenic characterization of a newly emerging porcine circovirus type 2b mutant first isolated in cases of vaccine failure in Korea
    Seo, HW; Park, C; Kang, I; Choi, K; Jeong, J; Park, SJ; Chae, C
  • Pathological findings associated with naturally acquired porcine circovirus type 2 associated disease
    Segalés, J; Rosell, C; Domingo, M
  • Tripping over emerging pathogens around the world: a phylogeographical approach for determining the epidemiology of Porcine circovirus-2 (PCV-2), considering global trading
    Vidigal, PMP; Mafra, CL; Silva, FMF; Fietto, JLR; Silva Júnior, A; Almeida, MR

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