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A Novel Zebrafish (Danio rerio) Assay for Assessing Musk Ambrette-Induced Toxicity

A Novel Zebrafish (Danio rerio) Assay for Assessing Musk Ambrette-Induced Toxicity Musk ambrette (4-tert-butyl-3-methoxy-2,6-dinitrotoluene) is a nitro musk, a cheap substitute for natural musk and a potential environmental pollutant based on its persistence, accumulation in human organisms. We investigated the acute toxicity of musk ambrette using wild-type AB and transgenic Tg(fli1a:EGFP) y1 zebrafish. Different concentrations were delivered to zebrafish by direct soaking from 6 to 72 h post-fertilization (hpf). The LC50 of musk ambrette was 76.4 µg mL−1. As musk ambrette concentration increased, zebrafish embryos showed developmental delays (50 µg mL−1, 22 hpf), pericardial edema (5 µg mL−1, 48 hpf), circulatory disturbances, curved body axis (1 µg mL−1, 72 hpf) and death (100 µg mL−1, 22 hpf). Target organ toxicity was evaluated by a zebrafish angiogenesis model. Musk ambrette induced cardiovascular morphological changes, vessel permeability variation, angiogenic changes and cardiotoxicity (10 µg mL−1, 48 hpf). The disappearance of caudal vein plexus confirmed the vascular development toxicity. Musk ambrette negatively affects early life-stage survival and demonstrates various toxicities in zebrafish. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Bulletin of Environmental Contamination and Toxicology Springer Journals

A Novel Zebrafish (Danio rerio) Assay for Assessing Musk Ambrette-Induced Toxicity

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Environment; Pollution, general; Environmental Health; Ecotoxicology; Soil Science & Conservation; Environmental Chemistry; Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution
ISSN
0007-4861
eISSN
1432-0800
DOI
10.1007/s00128-018-2363-z
pmid
29846757
Publisher site
See Article on Publisher Site

Abstract

Musk ambrette (4-tert-butyl-3-methoxy-2,6-dinitrotoluene) is a nitro musk, a cheap substitute for natural musk and a potential environmental pollutant based on its persistence, accumulation in human organisms. We investigated the acute toxicity of musk ambrette using wild-type AB and transgenic Tg(fli1a:EGFP) y1 zebrafish. Different concentrations were delivered to zebrafish by direct soaking from 6 to 72 h post-fertilization (hpf). The LC50 of musk ambrette was 76.4 µg mL−1. As musk ambrette concentration increased, zebrafish embryos showed developmental delays (50 µg mL−1, 22 hpf), pericardial edema (5 µg mL−1, 48 hpf), circulatory disturbances, curved body axis (1 µg mL−1, 72 hpf) and death (100 µg mL−1, 22 hpf). Target organ toxicity was evaluated by a zebrafish angiogenesis model. Musk ambrette induced cardiovascular morphological changes, vessel permeability variation, angiogenic changes and cardiotoxicity (10 µg mL−1, 48 hpf). The disappearance of caudal vein plexus confirmed the vascular development toxicity. Musk ambrette negatively affects early life-stage survival and demonstrates various toxicities in zebrafish.

Journal

Bulletin of Environmental Contamination and Toxicology – Springer Journals

Published: May 30, 2018

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