A novel SR protein binding site in a cis -regulatory element of HIV-1

A novel SR protein binding site in a cis -regulatory element of HIV-1 We have previously described a cis enhancing sequence (CES) in the gp41 region of the HIV-1 env gene. It could enhance HIV-1 Gag expression via both rev-dependent and CTE (constitutive transport element)-dependent pathways. We identified a functionally important and conserved region in the CES that contained a predicted binding sequence for an SR protein, ASF/SF2. We show here that ASF/SF2 bound to this sequence in an electrophoretic mobility shift assay and that the putative ASF/SF2-binding sequence was required for the enhancement of Gag expression by CES and might play a role in HIV-1 posttranscriptional regulation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

A novel SR protein binding site in a cis -regulatory element of HIV-1

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Springer Vienna
Copyright © 2010 by Springer-Verlag
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
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