Planta (2017) 246:579–584 DOI 10.1007/s00425-017-2743-3 SHORT COMMUNICATION A novel plant DNA extraction method using ﬁlter paper-based 96-well spin plate 1 1 Rui Shi Dilip R. Panthee Received: 23 May 2017 / Accepted: 10 July 2017 / Published online: 13 July 2017 Springer-Verlag GmbH Germany 2017 Abstract Introduction Main conclusion A simple, low cost and safe method using homemade ﬁlter paper-based 96-well spin plates Molecular markers have been developed in crops and veg- and homemade solutions was developed for high etables for genetic mapping and characterization of associated throughput plant DNA extraction to use in molecular genes and quantitative trait loci (QTLs). DNA-based molec- marker analysis. ular markers are neutral in phenotypic reaction and can be detected at any growth stage. Therefore, they are widely uti- A low cost and safe method was developed for high lized for marker-assisted selection (MAS) in breeding pro- throughput extraction of plant DNA for molecular marker grams for traits related to agronomic, horticultural, fruit analysis. In this method, we describe a simple way to quality, disease resistance, and others (Foolad and Panthee prepare 96-well spin plate using ﬁlter paper, a plant 2012). Availability of whole genome sequences and recent material product for DNA binding.
Planta – Springer Journals
Published: Jul 13, 2017
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