A novel lipoxygenase gene from developing rice seeds confers dual position specificity and responds to wounding and insect attack

A novel lipoxygenase gene from developing rice seeds confers dual position specificity and... OsLOX1 is a novel full-length cDNA isolated from developing rice seeds. We have examined its biochemical properties and expression patterns. The protein has dual positional specificity, as it releases both C-9 and C-13 oxidized products in a 4:3 ratio. OsLOX1 transcripts were detected at low abundance in immature seeds and newly germinated seedlings, but accumulate rapidly and transiently in response to wounding or brown planthopper (BPH) attack, reaching a peak 3 h after wounding and 6 h after insect feeding. We produced transgenic rice lines carrying either sense or antisense constructs under the control of a cauliflower mosaic virus 35S promoter, and these rice lines showed altered OsLOX1 activity. In all of the antisense lines and more than half of the sense lines the expression levels of OsLOX1, the levels of enzyme activity, and the levels of the endogenous OsLOX1 products (jasmonic acid, (Z)-3-hexenal and colneleic acid) at 6, 48, and 48 h after BPH feeding respectively, were below the levels found in non-transgenic control plants; yet, the levels in the remaining sense transformants were enhanced relative to controls. Transformants with a lower level of OsLOX1 expression were less able to tolerate BPH attack, while those with enhanced OsLOX1 expression were more resistant. Our data suggest that the OsLOX1 product is involved in tolerance of the rice plant to wounding and BPH attack. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

A novel lipoxygenase gene from developing rice seeds confers dual position specificity and responds to wounding and insect attack

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Publisher
Springer Netherlands
Copyright
Copyright © 2008 by Springer Science+Business Media B.V.
Subject
Life Sciences; Plant Pathology; Biochemistry, general; Plant Sciences
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-007-9278-0
Publisher site
See Article on Publisher Site

Abstract

OsLOX1 is a novel full-length cDNA isolated from developing rice seeds. We have examined its biochemical properties and expression patterns. The protein has dual positional specificity, as it releases both C-9 and C-13 oxidized products in a 4:3 ratio. OsLOX1 transcripts were detected at low abundance in immature seeds and newly germinated seedlings, but accumulate rapidly and transiently in response to wounding or brown planthopper (BPH) attack, reaching a peak 3 h after wounding and 6 h after insect feeding. We produced transgenic rice lines carrying either sense or antisense constructs under the control of a cauliflower mosaic virus 35S promoter, and these rice lines showed altered OsLOX1 activity. In all of the antisense lines and more than half of the sense lines the expression levels of OsLOX1, the levels of enzyme activity, and the levels of the endogenous OsLOX1 products (jasmonic acid, (Z)-3-hexenal and colneleic acid) at 6, 48, and 48 h after BPH feeding respectively, were below the levels found in non-transgenic control plants; yet, the levels in the remaining sense transformants were enhanced relative to controls. Transformants with a lower level of OsLOX1 expression were less able to tolerate BPH attack, while those with enhanced OsLOX1 expression were more resistant. Our data suggest that the OsLOX1 product is involved in tolerance of the rice plant to wounding and BPH attack.

Journal

Plant Molecular BiologySpringer Journals

Published: Jan 5, 2008

References

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