Arch Virol (2004) 149: 1107–1116
A new potyvirus from tuberose (Polianthes tuberosa)
, H.-Y. Zheng
, J. Chen
, J.-P. Chen
, Q.-Y. Zhang
, J. F. Antoniw
, and M. J. Adams
Department of Virology and Biotechnology, Zhejiang Academy
of Agricultural Sciences, Hangzhou, P.R. China
Plant Pathogen Interactions Division, Rothamsted Research,
Harpenden, Herts, U.K.
Received September 29, 2003; accepted December 17, 2003
Published online March 1, 2004
Summary. Tuberose plants with mild mottle symptoms, growing in a glasshouse
in Hangzhou, China, contained virions and inclusion bodies typical of a potyvirus.
The virus was mechanically transmitted to tuberose but not to 14 other test plant
species. A fragment of 4607 nucleotides, corresponding to the 3
-half of a typical
potyvirus was ampliﬁed by RT-PCR using degenerate primers and sequenced. The
most similar sequence in the databases was that of Tuberose mild mosaic virus
(TuMMV) from Taiwan and this was the only virus signiﬁcantly related to it in
phylogenetic analyses. The new sequence had 71.1% nt and 76.6% aa identity
to TuMMV in the coat protein. Western blot analyses using antisera raised to
expressed coat protein showed that the two viruses were serologically related.
Although there are no substantial biological data to distinguish the Hangzhou
isolate from TuMMV, the molecular difference between the two virus isolates is
similar to, or slightly greater than, that between several pairs of well-established
potyvirus species. These results therefore suggest that the Hangzhou isolate should
be regarded as a new member of the genus Potyvirus, and we have tentatively
named it Tuberose mild mottle virus.
Tuberose (Polianthes tuberosa L.), family Agavaceae, is a tender, tall, slim peren-
nial with long slender leaves, a tuberous root and large, very fragrant, white lily-
like ﬂowers which grows wild in CentralAmerica. It is cultivated all over the world
for the cut ﬂower trade and also in Egypt, China, France and Morocco for its oil.
The ﬁrst two authors contributed equally to this study.