A new ilarvirus isolated from Viola × wittrockiana and its detection in pansy germoplasm by qRT-PCR

A new ilarvirus isolated from Viola × wittrockiana and its detection in pansy germoplasm by qRT-PCR An infectious agent was transmitted mechanically from samples of Viola spp. showing white mosaic and leaf deformation to Nicotiana benthamiana . dsRNA extracted from the N. benthamiana plants migrated as four specific bands that were absent in non-inoculated plants. Sequence analysis of cDNA clones generated from the second-smallest dsRNA showed the greatest similarity to the RNA3 of prune dwarf virus (PDV) (genus Ilarvirus , family Bromoviridae ). However, because of differences in molecular, biological, and serological properties between this virus isolate and PDV, a new ilarvirus species, named “Viola white distortion associated virus” (VWDaV) is proposed. Specific oligonucleotides and a TaqMan ® probe were designed for diagnostic purposes. The possible association between the virus and the original white distortion symptoms is discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

A new ilarvirus isolated from Viola × wittrockiana and its detection in pansy germoplasm by qRT-PCR

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Publisher
Springer Journals
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1837-x
Publisher site
See Article on Publisher Site

Abstract

An infectious agent was transmitted mechanically from samples of Viola spp. showing white mosaic and leaf deformation to Nicotiana benthamiana . dsRNA extracted from the N. benthamiana plants migrated as four specific bands that were absent in non-inoculated plants. Sequence analysis of cDNA clones generated from the second-smallest dsRNA showed the greatest similarity to the RNA3 of prune dwarf virus (PDV) (genus Ilarvirus , family Bromoviridae ). However, because of differences in molecular, biological, and serological properties between this virus isolate and PDV, a new ilarvirus species, named “Viola white distortion associated virus” (VWDaV) is proposed. Specific oligonucleotides and a TaqMan ® probe were designed for diagnostic purposes. The possible association between the virus and the original white distortion symptoms is discussed.

Journal

Archives of VirologySpringer Journals

Published: Mar 1, 2014

References

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