A mutation in the gene for polynucleotide kinase of bacteriophage T4 K10 affects mRNA processing

A mutation in the gene for polynucleotide kinase of bacteriophage T4 K10 affects mRNA processing The bacteriophage T4 insertion-substitution (I/S) vector system has become one of the most important tools for the introduction of site-directed mutations into the T4 genome. In this study, we show that the I/S phage T4 K10 carries two point mutations within the gene for polynucleotide kinase pseT , resulting in amino acid substitutions G14D and R229H. The G14D mutation impairs 5′-kinase activity in vivo as well as in vitro and leads to diminished processing at secondary sites of several RegB-cleaved transcripts. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

A mutation in the gene for polynucleotide kinase of bacteriophage T4 K10 affects mRNA processing

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Publisher
Springer Journals
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1800-x
Publisher site
See Article on Publisher Site

Abstract

The bacteriophage T4 insertion-substitution (I/S) vector system has become one of the most important tools for the introduction of site-directed mutations into the T4 genome. In this study, we show that the I/S phage T4 K10 carries two point mutations within the gene for polynucleotide kinase pseT , resulting in amino acid substitutions G14D and R229H. The G14D mutation impairs 5′-kinase activity in vivo as well as in vitro and leads to diminished processing at secondary sites of several RegB-cleaved transcripts.

Journal

Archives of VirologySpringer Journals

Published: Feb 1, 2014

References

  • Bacteriophage T4 genome
    Miller, E; Kutter, E; Mosig, G; Arisaka, F; Kunisawa, T; Rüger, W
  • Bacteriophage T4 host range is expanded by duplications of a small domain of the tail fiber adhesin
    Tetart, F; Repoila, F; Monod, C; Krisch, HM
  • Genome plasticity in the distal tail fiber locus of the T-even bacteriophage: recombination between conserved motifs swaps adhesin specificity
    Tetart, F; Desplats, C; Krisch, HM

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