The bacteriophage T4 insertion-substitution (I/S) vector system has become one of the most important tools for the introduction of site-directed mutations into the T4 genome. In this study, we show that the I/S phage T4 K10 carries two point mutations within the gene for polynucleotide kinase pseT , resulting in amino acid substitutions G14D and R229H. The G14D mutation impairs 5′-kinase activity in vivo as well as in vitro and leads to diminished processing at secondary sites of several RegB-cleaved transcripts.
Archives of Virology – Springer Journals
Published: Feb 1, 2014
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