A multiplex RT-PCR for simultaneous detection and identification of five viruses and two viroids infecting chrysanthemum

A multiplex RT-PCR for simultaneous detection and identification of five viruses and two viroids... Pathogens causing significant economic losses in chrysanthemum include tomato aspermy virus (TAV), chrysanthemum virus B (CVB), cucumber mosaic virus (CMV), tobacco mosaic virus (TMV), potato virus Y (PVY), chrysanthemum stunt viroid (CSVd) and chrysanthemum chlorotic mottle viroid (CChMVd). A multiplex reverse transcription polymerase chain reaction (RT-PCR) method, using specific primer sets for each virus or viroid, was developed for simultaneous detection and differentiation of TAV, CVB, CMV, TMV, PVY, CChMVd, and CSVd. The RT-PCR method was validated by testing chrysanthemum samples collected from different regions of China. In this study, CVB, TAV, TMV, PVY, CSVd, CMV, and CChMVd were detected, respectively, in 24.7 %, 17.5 %, 4.4 %, 4.4 %, 2.9 %, 2.5 %, and 1.5 % of the samples tested. These results indicate that CVB and TAV (24.7 % and 17.5 %) are common, whereas CMV, TMV, CChMVd, CSVd, and PVY (all below 5 %) are less frequently encountered. This new multiplex RT-PCR method has potential to be used routinely in large-scale virus and viroid surveys. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

A multiplex RT-PCR for simultaneous detection and identification of five viruses and two viroids infecting chrysanthemum

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Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2360-z
Publisher site
See Article on Publisher Site

Abstract

Pathogens causing significant economic losses in chrysanthemum include tomato aspermy virus (TAV), chrysanthemum virus B (CVB), cucumber mosaic virus (CMV), tobacco mosaic virus (TMV), potato virus Y (PVY), chrysanthemum stunt viroid (CSVd) and chrysanthemum chlorotic mottle viroid (CChMVd). A multiplex reverse transcription polymerase chain reaction (RT-PCR) method, using specific primer sets for each virus or viroid, was developed for simultaneous detection and differentiation of TAV, CVB, CMV, TMV, PVY, CChMVd, and CSVd. The RT-PCR method was validated by testing chrysanthemum samples collected from different regions of China. In this study, CVB, TAV, TMV, PVY, CSVd, CMV, and CChMVd were detected, respectively, in 24.7 %, 17.5 %, 4.4 %, 4.4 %, 2.9 %, 2.5 %, and 1.5 % of the samples tested. These results indicate that CVB and TAV (24.7 % and 17.5 %) are common, whereas CMV, TMV, CChMVd, CSVd, and PVY (all below 5 %) are less frequently encountered. This new multiplex RT-PCR method has potential to be used routinely in large-scale virus and viroid surveys.

Journal

Archives of VirologySpringer Journals

Published: May 1, 2015

References

  • Occurrence of Turnip mosaic virus on ornamental crops in Iran
    Farzadfar, Sh; Ohshima, K; Pourrahim, R; Golnaraghi, AR; Jalali, S; Ahoonmanesh, A
  • A multiplex RT-PCR for rapid and simultaneous detection of viruses and viroids in chrysanthemum
    Song, A; You, Y; Chen, F; Li, P; Jiang, J; Chen, S
  • Coat protein gene diversity among Chrysanthemum virus B isolates from India
    Singh, L; Hallan, V; Jabeen, N; Singh, AK; Ram, R; Martin, DP; Zaidi, AA
  • Elimination of mixed infection of Cucumber mosaic and Tomato aspermy virus from chrysanthemum morifolium Ramat. cv. Pooja by shoot meristem culture
    Kumar, S; Khan, MS; Raj, SK; Sharma, AK

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