A multi-responsive gene encoding 1-aminocyclopropane-1- carboxylate synthase (ACS6) in mature Arabidopsis leaves

A multi-responsive gene encoding 1-aminocyclopropane-1- carboxylate synthase (ACS6) in mature... Physiological and biochemical studies have provided evidence that mechanical strain (touch)-induced modifications in plant growth and development may be due to ethylene. In order to better understand the involvement of ethylene in touch-induced responses, we identified and characterized an Arabidopsis cDNA (ACS6) encoding 1-aminocyclopropane-1-carboxylic acid (ACC) synthase which is an important regulatory enzyme in the ethylene biosynthetic pathway. Northern analysis showed that ACS6 was induced by touch in the leaves of 3-week old light-grown plants within 5 min and reached maximum transcription at 15 min. ACC, which is the product of ACC synthase and the immediate precursor to ethylene, exhibited a dramatic rise between 15 and 30 min after touch stimulation. Experiments with multiple touch treatments showed that a saturation in gene expression was obtained with one touch treatment and subsequent touch stimulations were progressively less effective in promoting ACS6 expression. Additional characterization of ACS6 gene expression indicated that the gene is also induced by wounding, and by treatment with LiCl, NaCl, CuCl2, auxin, cycloheximide (CHX), aminooxyacetic acid (AOA) and ethylene. ACC levels were also increased in response to each of these treatments with the exception of CHX and AOA which resulted in a decrease and no effect, respectively. Our results show that ACS6 is rapidly turned on in response to touch which is followed by an increase in ACC which is the immediate precursor to ethylene, thereby providing evidence that it is responsible for touch-inducible ethylene production in light-grown Arabidopsis plants. The identification and characterization of ACS6 now provides us with a tool to better understand the involvement of ethylene produced in response to external stimuli as well as during plant growth and development. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

A multi-responsive gene encoding 1-aminocyclopropane-1- carboxylate synthase (ACS6) in mature Arabidopsis leaves

Loading next page...
 
/lp/springer_journal/a-multi-responsive-gene-encoding-1-aminocyclopropane-1-carboxylate-coMLelbNxD
Publisher
Kluwer Academic Publishers
Copyright
Copyright © 1999 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1006177902093
Publisher site
See Article on Publisher Site

Abstract

Physiological and biochemical studies have provided evidence that mechanical strain (touch)-induced modifications in plant growth and development may be due to ethylene. In order to better understand the involvement of ethylene in touch-induced responses, we identified and characterized an Arabidopsis cDNA (ACS6) encoding 1-aminocyclopropane-1-carboxylic acid (ACC) synthase which is an important regulatory enzyme in the ethylene biosynthetic pathway. Northern analysis showed that ACS6 was induced by touch in the leaves of 3-week old light-grown plants within 5 min and reached maximum transcription at 15 min. ACC, which is the product of ACC synthase and the immediate precursor to ethylene, exhibited a dramatic rise between 15 and 30 min after touch stimulation. Experiments with multiple touch treatments showed that a saturation in gene expression was obtained with one touch treatment and subsequent touch stimulations were progressively less effective in promoting ACS6 expression. Additional characterization of ACS6 gene expression indicated that the gene is also induced by wounding, and by treatment with LiCl, NaCl, CuCl2, auxin, cycloheximide (CHX), aminooxyacetic acid (AOA) and ethylene. ACC levels were also increased in response to each of these treatments with the exception of CHX and AOA which resulted in a decrease and no effect, respectively. Our results show that ACS6 is rapidly turned on in response to touch which is followed by an increase in ACC which is the immediate precursor to ethylene, thereby providing evidence that it is responsible for touch-inducible ethylene production in light-grown Arabidopsis plants. The identification and characterization of ACS6 now provides us with a tool to better understand the involvement of ethylene produced in response to external stimuli as well as during plant growth and development.

Journal

Plant Molecular BiologySpringer Journals

Published: Sep 29, 2004

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off