Mammahan Genome 8, 143-145 (1997). ellome 9 Spnnger Verlag New York lnc 1997 A microsatellite map of the pink-eyed dilution (p) deletion complex in mouse Chromosome 7 M.S. Dhar, 1 D.K. Johnson z 1University of Tennessee Graduate School of Biomedical Sciences, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-8077, USA 2Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-8077, USA Received: 27 June 1996 / Accepted: 4 October 1996 The pink-eyed dilution (p) locus in mouse Chromosome (Chr) 7 is Taq polymerase (Fisher Scientific, Pittsburgh, PA and Glbco BRL one of the seven loci used as markers for experimental mutagen- Life Technologies, Inc., Gaithersburg, MD). Reactions were car- esis in the specific locus test (Russell 1951). The p locus appears ried out in a GeneAmp PCR System 9600 (Perkin-Elmer Corp.) as to encode an integral membrane transporter protein that may be follows: initial denaturation for 5 rain at 94~ was followed by 30 involved in the transport of tyrosine. Mutations in this gene are cycles of 94~ denaturation for 45 s, anneahng at 50-60~ for 45 associated with melanin reduction in the skin, hair, and eyes s, and extension for 2 rain 30 s at 72~ and finally
Mammalian Genome – Springer Journals
Published: Mar 24, 2009
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