Mammalian Genome 10, 1106–1107 (1999). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1999 A locus responsible for hypogonadism (hgn) is located on rat Chromosome 10 1 1 1 2 1 Hiroetsu Suzuki, Michiko Kokado, Kenichi Saito, Tetsuo Kunieda, Katsushi Suzuki Department of Veterinary Physiology, Nippon Veterinary and Animal Science University, 1-7-1 Kyonano-cho, Musashino-shi, Tokyo 180-0023, Japan Faculty of Agriculture, Okayama University, Okayama, Okayama 7000–8532, Japan Received: 5 February 1999 / Accepted: 21 July 1999 Hypogonadic rats of the HGN inbred strain show male sterility Research Genetics Inc. (Huntsville, Ala.). The PCR was carried controlled by an autosomal single recessive allele (hgn). The testis out in 10 m1 of a reaction mixture containing 25 ng of the genomic weight of adult hgn/hgn rats is about 1% that of normal rats, and DNA, 1.0 pmol of each of the oligonucleotide primers, 2.0 nmol of only a few thin seminiferous tubules surrounded by islet-like con- each dNTP, and 0.26 U of Taq polymerase (TaKaRa) in the reac- formations of Leydig cells are present in the fibrous interstitial tion buffer recommended by the manufacturer. After initial dena- tissue of the testis (Suzuki et al. 1988). Primordial germ cells do turation for 180
Mammalian Genome – Springer Journals
Published: Nov 1, 1999
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