A large-fragment porcine genomic library resource in a BAC vector

A large-fragment porcine genomic library resource in a BAC vector Mammalian Genome 11, 811–814 (2000). DOI: 10.1007/s003350010155 Incorporating Mouse Genome © Springer-Verlag New York Inc. 2000 1 1 2 1 Susan I. Anderson, Nestor L. Lopez-Corrales, Barbara Gorick, Alan L. Archibald Roslin Institute (Edinburgh), Roslin, Midlothian EH25 9PS, Scotland, UK HGMP Resource Center, Hinxton, Cambridge CB10 1SB, UK Received: 4 January 2000 / Accepted: 6 May 2000 Genome scanning experiments in pigs are defining quantitative cessfully screened by both methods. We report here on the char- trait loci (QTL) which contribute to the genetic variation in traits acteristics of this pig BAC library. of economic or biological importance (Andersson et al. 1994; The vector pBeloBAC11 was digested with HindIII and de- Walling et al. 1998). Although markers flanking QTL can be used phosphorylated with shrimp alkaline phosphatase (Boehringer, for marker-assisted selection, significant extra benefits will accrue Lewes, East Sussex, UK). Cut vector was separated from residual from the isolation of the trait genes from within the QTL. These uncut vector by electrophoresis through low melting point (LMP) benefits include the characterization of desirable (and undesirable) agarose (FMC, BioProducts, Rockland, ME) and isolated after trait alleles and the development of allele-specific diagnostic tests. agarase treatment (New England Biolabs, Beverly, MA). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

A large-fragment porcine genomic library resource in a BAC vector

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Publisher
Springer-Verlag
Copyright
Copyright © 2000 by Springer-Verlag New York Inc.
Subject
Life Sciences; Cell Biology; Anatomy; Zoology
ISSN
0938-8990
eISSN
1432-1777
D.O.I.
10.1007/s003350010155
Publisher site
See Article on Publisher Site

Abstract

Mammalian Genome 11, 811–814 (2000). DOI: 10.1007/s003350010155 Incorporating Mouse Genome © Springer-Verlag New York Inc. 2000 1 1 2 1 Susan I. Anderson, Nestor L. Lopez-Corrales, Barbara Gorick, Alan L. Archibald Roslin Institute (Edinburgh), Roslin, Midlothian EH25 9PS, Scotland, UK HGMP Resource Center, Hinxton, Cambridge CB10 1SB, UK Received: 4 January 2000 / Accepted: 6 May 2000 Genome scanning experiments in pigs are defining quantitative cessfully screened by both methods. We report here on the char- trait loci (QTL) which contribute to the genetic variation in traits acteristics of this pig BAC library. of economic or biological importance (Andersson et al. 1994; The vector pBeloBAC11 was digested with HindIII and de- Walling et al. 1998). Although markers flanking QTL can be used phosphorylated with shrimp alkaline phosphatase (Boehringer, for marker-assisted selection, significant extra benefits will accrue Lewes, East Sussex, UK). Cut vector was separated from residual from the isolation of the trait genes from within the QTL. These uncut vector by electrophoresis through low melting point (LMP) benefits include the characterization of desirable (and undesirable) agarose (FMC, BioProducts, Rockland, ME) and isolated after trait alleles and the development of allele-specific diagnostic tests. agarase treatment (New England Biolabs, Beverly, MA).

Journal

Mammalian GenomeSpringer Journals

Published: Feb 13, 2014

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