A large-fragment porcine genomic library resource in a BAC vector

A large-fragment porcine genomic library resource in a BAC vector Mammalian Genome 11, 811–814 (2000). DOI: 10.1007/s003350010155 Incorporating Mouse Genome © Springer-Verlag New York Inc. 2000 1 1 2 1 Susan I. Anderson, Nestor L. Lopez-Corrales, Barbara Gorick, Alan L. Archibald Roslin Institute (Edinburgh), Roslin, Midlothian EH25 9PS, Scotland, UK HGMP Resource Center, Hinxton, Cambridge CB10 1SB, UK Received: 4 January 2000 / Accepted: 6 May 2000 Genome scanning experiments in pigs are defining quantitative cessfully screened by both methods. We report here on the char- trait loci (QTL) which contribute to the genetic variation in traits acteristics of this pig BAC library. of economic or biological importance (Andersson et al. 1994; The vector pBeloBAC11 was digested with HindIII and de- Walling et al. 1998). Although markers flanking QTL can be used phosphorylated with shrimp alkaline phosphatase (Boehringer, for marker-assisted selection, significant extra benefits will accrue Lewes, East Sussex, UK). Cut vector was separated from residual from the isolation of the trait genes from within the QTL. These uncut vector by electrophoresis through low melting point (LMP) benefits include the characterization of desirable (and undesirable) agarose (FMC, BioProducts, Rockland, ME) and isolated after trait alleles and the development of allele-specific diagnostic tests. agarase treatment (New England Biolabs, Beverly, MA). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

A large-fragment porcine genomic library resource in a BAC vector

Loading next page...
 
/lp/springer_journal/a-large-fragment-porcine-genomic-library-resource-in-a-bac-vector-LpXhHfxyal
Publisher
Springer-Verlag
Copyright
Copyright © 2000 by Springer-Verlag New York Inc.
Subject
Life Sciences; Cell Biology; Anatomy; Zoology
ISSN
0938-8990
eISSN
1432-1777
D.O.I.
10.1007/s003350010155
Publisher site
See Article on Publisher Site

Abstract

Mammalian Genome 11, 811–814 (2000). DOI: 10.1007/s003350010155 Incorporating Mouse Genome © Springer-Verlag New York Inc. 2000 1 1 2 1 Susan I. Anderson, Nestor L. Lopez-Corrales, Barbara Gorick, Alan L. Archibald Roslin Institute (Edinburgh), Roslin, Midlothian EH25 9PS, Scotland, UK HGMP Resource Center, Hinxton, Cambridge CB10 1SB, UK Received: 4 January 2000 / Accepted: 6 May 2000 Genome scanning experiments in pigs are defining quantitative cessfully screened by both methods. We report here on the char- trait loci (QTL) which contribute to the genetic variation in traits acteristics of this pig BAC library. of economic or biological importance (Andersson et al. 1994; The vector pBeloBAC11 was digested with HindIII and de- Walling et al. 1998). Although markers flanking QTL can be used phosphorylated with shrimp alkaline phosphatase (Boehringer, for marker-assisted selection, significant extra benefits will accrue Lewes, East Sussex, UK). Cut vector was separated from residual from the isolation of the trait genes from within the QTL. These uncut vector by electrophoresis through low melting point (LMP) benefits include the characterization of desirable (and undesirable) agarose (FMC, BioProducts, Rockland, ME) and isolated after trait alleles and the development of allele-specific diagnostic tests. agarase treatment (New England Biolabs, Beverly, MA).

Journal

Mammalian GenomeSpringer Journals

Published: Feb 13, 2014

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off