A hybrid co-culture model with endothelial cells designed for the hepatic tissue engineering

A hybrid co-culture model with endothelial cells designed for the hepatic tissue engineering losing their characteristic morphologies and biological functions. To avoid this problem, a hybrid co-culture sys- tem was proposed to elucidate the effect of cellular com- munication on the phenotype of hepatic cells. A monolayer of endothelial cells (ECs) was co-cultured on the surface of a three-dimensional (3D) scaffold embedded with HepG2 cells. In this hybrid co-culture system, the growth of encapsulated hepatic cells is barely influenced by the co- cultured ECs. However, the liver-special functions of hepatic cells, including the albumin secretion and the expression levels of hepatocyte-specific genes, are sig- nificantly improved. It is deduced that the improved liver- special functions is likely related to the paracrine mechan- isms. Hence, this hybrid co-culture model may open a window for the co-cultivation of the multi-type of cells as well as the study of cell-cell signaling interaction. * Xiaobo Huang huangtyut@163.com * Bin Tang 1 Introduction tangbin@tyut.edu.cn Hepatic tissue engineering is an attractive strategy aiming at Research Institute of Surface Engineering, Taiyuan University of Technology, Taiyuan 030024, China developing a composite tissue construct for the liver 139 Page 2 of 8 J Mater Sci: Mater Med (2017) 28:139 therapeutic replacement [1–3]. The formed tissue constructs Dulbecco’s Modified Eagle’s Medium (DMEM) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Materials Science: Materials in Medicine Springer Journals

A hybrid co-culture model with endothelial cells designed for the hepatic tissue engineering

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Publisher
Springer US
Copyright
Copyright © 2017 by Springer Science+Business Media, LLC
Subject
Materials Science; Biomaterials; Biomedical Engineering; Regenerative Medicine/Tissue Engineering; Polymer Sciences; Ceramics, Glass, Composites, Natural Materials; Surfaces and Interfaces, Thin Films
ISSN
0957-4530
eISSN
1573-4838
D.O.I.
10.1007/s10856-017-5950-3
Publisher site
See Article on Publisher Site

Abstract

losing their characteristic morphologies and biological functions. To avoid this problem, a hybrid co-culture sys- tem was proposed to elucidate the effect of cellular com- munication on the phenotype of hepatic cells. A monolayer of endothelial cells (ECs) was co-cultured on the surface of a three-dimensional (3D) scaffold embedded with HepG2 cells. In this hybrid co-culture system, the growth of encapsulated hepatic cells is barely influenced by the co- cultured ECs. However, the liver-special functions of hepatic cells, including the albumin secretion and the expression levels of hepatocyte-specific genes, are sig- nificantly improved. It is deduced that the improved liver- special functions is likely related to the paracrine mechan- isms. Hence, this hybrid co-culture model may open a window for the co-cultivation of the multi-type of cells as well as the study of cell-cell signaling interaction. * Xiaobo Huang huangtyut@163.com * Bin Tang 1 Introduction tangbin@tyut.edu.cn Hepatic tissue engineering is an attractive strategy aiming at Research Institute of Surface Engineering, Taiyuan University of Technology, Taiyuan 030024, China developing a composite tissue construct for the liver 139 Page 2 of 8 J Mater Sci: Mater Med (2017) 28:139 therapeutic replacement [1–3]. The formed tissue constructs Dulbecco’s Modified Eagle’s Medium (DMEM)

Journal

Journal of Materials Science: Materials in MedicineSpringer Journals

Published: Aug 10, 2017

References

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