A hybrid co-culture model with endothelial cells designed for the hepatic tissue engineering

A hybrid co-culture model with endothelial cells designed for the hepatic tissue engineering losing their characteristic morphologies and biological functions. To avoid this problem, a hybrid co-culture sys- tem was proposed to elucidate the effect of cellular com- munication on the phenotype of hepatic cells. A monolayer of endothelial cells (ECs) was co-cultured on the surface of a three-dimensional (3D) scaffold embedded with HepG2 cells. In this hybrid co-culture system, the growth of encapsulated hepatic cells is barely influenced by the co- cultured ECs. However, the liver-special functions of hepatic cells, including the albumin secretion and the expression levels of hepatocyte-specific genes, are sig- nificantly improved. It is deduced that the improved liver- special functions is likely related to the paracrine mechan- isms. Hence, this hybrid co-culture model may open a window for the co-cultivation of the multi-type of cells as well as the study of cell-cell signaling interaction. * Xiaobo Huang huangtyut@163.com * Bin Tang 1 Introduction tangbin@tyut.edu.cn Hepatic tissue engineering is an attractive strategy aiming at Research Institute of Surface Engineering, Taiyuan University of Technology, Taiyuan 030024, China developing a composite tissue construct for the liver 139 Page 2 of 8 J Mater Sci: Mater Med (2017) 28:139 therapeutic replacement [1–3]. The formed tissue constructs Dulbecco’s Modified Eagle’s Medium (DMEM) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Materials Science: Materials in Medicine Springer Journals

A hybrid co-culture model with endothelial cells designed for the hepatic tissue engineering

Loading next page...
 
/lp/springer_journal/a-hybrid-co-culture-model-with-endothelial-cells-designed-for-the-ri2XZ1vwao
Publisher
Springer US
Copyright
Copyright © 2017 by Springer Science+Business Media, LLC
Subject
Materials Science; Biomaterials; Biomedical Engineering; Regenerative Medicine/Tissue Engineering; Polymer Sciences; Ceramics, Glass, Composites, Natural Materials; Surfaces and Interfaces, Thin Films
ISSN
0957-4530
eISSN
1573-4838
D.O.I.
10.1007/s10856-017-5950-3
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial