A distinct tospovirus causing necrotic streak on Alstroemeria sp.
J. Th. J. Verhoeven
Received: 13 October 2009 / Accepted: 14 December 2009 / Published online: 12 February 2010
Ó The Author(s) 2010. This article is published with open access at Springerlink.com
Abstract A tospovirus causing necrotic streaks on leaves
was isolated from Alstroemeria sp. in Colombia. Infected
samples reacted positively with tomato spotted wilt virus
(TSWV) antiserum during preliminary serological tests.
Further analysis revealed a close serological relationship to
tomato chlorotic spot virus (TCSV) and groundnut ringspot
virus (GRSV). A major part of the S-RNA segment,
encompassing the nucleocapsid (N) protein gene, the 5
untranslated region and a part of the intergenic region 3
the N gene, was cloned and sequenced. The deduced N
protein sequence showed highest amino acid identity
(82%) to that of TCSV, indicating that the virus represents
a new tospovirus species, for which the name Alstroemeria
necrotic streak virus (ANSV) is coined. Phylogenetic
analysis based on the N protein sequence revealed that
this Alstroemeria-infecting tospovirus clustered with
tospoviruses from the American continent. Frankliniella
occidentalis was identiﬁed as potential vector species for
Colombia represents one of the most important countries
for production and export of various ranges of cut ﬂowers,
with annual sales over 475 million US $ . Worldwide,
Colombia has the largest Alstroemeria cultivation, with a
production area exceeding 200 ha (Ko
BV). Native to South America, Alstroemeria is becoming
an important ornamental plant worldwide . So far, at
least 12 viruses, belonging to the genera Carlavirus, Cu-
cumovirus, Fabavirus, Nepovirus, Potexvirus, Potyvirus,
Tobamovirus, Tobravirus and Tospovirus have been
reported to infect Alstroemeria sp. .
The genus Tospovirus contains the plant-infecting
members of the Bunyaviridae, a family of primarily ani-
mal-infecting viruses . The type member of this genus
is tomato spotted wilt virus (TSWV), which has been
studied extensively because of its economic impact and
broad host range [14, 15].
Tospovirus particles are quasi-spherical, 80–120 nm in
diameter and enveloped by a lipid membrane. They are
propagatively transmitted by thrips (Thysanoptera, Thrip-
idae), of which the western ﬂower thrips, Frankliniella
occidentalis (Pergande) is the most important vector spe-
cies of the 13 thrips species identiﬁed as a tospovirus
vector [9, 26, 28, 34].
Tospoviruses contain a single-stranded, tripartite RNA
genome [10, 11, 20] of which the small (S) RNA segment
encodes the nucleocapsid (N) protein and a nonstructural
protein in an ambisense gene arrangement.
So far, 19 tospovirus species are recognised  based on
nucleocapsid (N) protein sequence identity (90% threshold)
and vector speciﬁcity . Members of a few of them, i.e.
R. Goldbach: Deceased.
The nucleotide sequence data reported in this manuscript have been
deposited at the NCBI/GenBank under accession no. GQ478668.
A. Hassani-Mehraban Á J. Saaijer Á D. Peters Á
R. Kormelink (&)
Laboratory of Virology, Department of Plant Sciences,
Wageningen University, P.O. Box 629,
6700 AP Wageningen, The Netherlands
M. Botermans Á J. Th. J. Verhoeven
Section of Virology, Plant Protection Service,
P.O. Box 9102, 6700 HC Wageningen, The Netherlands
Naktuinbouw, P.O. Box 40, 2370 AA Roelofarendsveen,
Arch Virol (2010) 155:423–428