622 Mammalian Genome 8, Brief Data Reports Map position: Ddx4 is located near the distal end of mouse Chro- mosome (Chr) 13:proximal-D13Mit148-(1.1 cM + 1.1 SE)- Ddx4, Etfa, D13Mit196, D13Hun30-(1.1 + 1.1)-D13Mit35, D13Bir20-distal. Note that D13Mit35 marks the distal end of this Chr 13 map. Method of mapping: Ddx4 was mapped by use of The Jackson Laboratory BSS interspecific backcross DNA panel, which con- tains 94 N 2 animals from the cross (C57BL/6JEi x SPRET/Ei)F a x Fig. 1, (A) Genetic mapping of Loxl on mouse Chr 9. Haptotypes of Loxl. SPRET/Ei . Rab7, and D9Mit32 on mouse Chr 9 in 93 [C57BL/6J-bg~X (C57BL16J-bg ~ Database deposit information: MGD Accession Number: MGD- � CAST/EiJ)F~] intersubspecific backcross mice. Solid boxes represent the CREX-11291 homozygous C57BL/6J pattern, and open boxes, the F t pattern. The num- Allele detection: For chromosomal mapping, genomic DNAs were ber of mice of each haplotype is indicated. (B) Composite genetic map of PCR amplified with primers vg2;21mer (GTCCAAAAGT- part of mouse Chr 9 showing the relative position of the rough coat locus GACATATATAC), and vas3;21mer (GGTTGATCAGTTCTC- (re) and Loxl. Marker locations are expressed as genetic distance from the GAGTTG) derived from Ddx4 eDNA sequence. With this
Mammalian Genome – Springer Journals
Published: Apr 1, 2009
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