A convenient semi-quantitative method for the diagnosis of Epstein-Barr virus reactivation

A convenient semi-quantitative method for the diagnosis of Epstein-Barr virus reactivation A semi-quantitative determination of Epstein-Barr virus (EBV) viremia has been devised. Peripheral blood mononuclear cells are recovered by Ficoll gradient and numerated. Five μl aliquots of recovered cell suspension and 5 μl of two standard dilutions (containing 500 and 100 cells, respectively) are subjected to a nested polymerase chain reaction (PCR). This technique has been evaluated over 3 years for the follow-up of 45 patients attending the Bone Marrow Transplantation Unit of the “Centre Hospitalier et Universitaire de Nancy”. EBV reactivation was diagnosed in 13 patients (28%). Positivity of PCR for 100 cells was found in 9 patients of whom 6 developed lymphoma or lymphoproliferative disorder. This technique is easy to perform and doesn’t necessitate any specific material besides the one necessary for routine genic amplification. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

A convenient semi-quantitative method for the diagnosis of Epstein-Barr virus reactivation

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Publisher
Springer-Verlag
Copyright
Copyright © 2000 by Springer-Verlag/Wien
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050070051
Publisher site
See Article on Publisher Site

Abstract

A semi-quantitative determination of Epstein-Barr virus (EBV) viremia has been devised. Peripheral blood mononuclear cells are recovered by Ficoll gradient and numerated. Five μl aliquots of recovered cell suspension and 5 μl of two standard dilutions (containing 500 and 100 cells, respectively) are subjected to a nested polymerase chain reaction (PCR). This technique has been evaluated over 3 years for the follow-up of 45 patients attending the Bone Marrow Transplantation Unit of the “Centre Hospitalier et Universitaire de Nancy”. EBV reactivation was diagnosed in 13 patients (28%). Positivity of PCR for 100 cells was found in 9 patients of whom 6 developed lymphoma or lymphoproliferative disorder. This technique is easy to perform and doesn’t necessitate any specific material besides the one necessary for routine genic amplification.

Journal

Archives of VirologySpringer Journals

Published: Oct 1, 2000

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