A conserved zinc finger motif in the coat protein of Tomato leaf curl Bangalore virus is responsible for binding to ssDNA

A conserved zinc finger motif in the coat protein of Tomato leaf curl Bangalore virus is... Tomato leaf curl Bangalore virus (ToLCBV-(Ban5)) from south India belongs to the genus Begomovirus of the family Geminiviridae . So far, only the DNA A component has been identified in this virus. In this paper we report the overexpression of ToLCBV-(Ban5) coat protein (CP) in E. coli and its characterization. The purified recombinant CP bound preferentially to ssDNA in a sequence non-specific manner. Deletion of 50 amino acids from the N-terminus, including a putative N-terminal α helix, did not result in the loss of binding to DNA. A search for motifs responsible for DNA binding indicated a conserved putative zinc finger motif in the CPs (corresponding to residues 65–85 in ToLCBV-(Ban 5) CP) of begomoviruses. The role of this motif in DNA binding was evaluated through site-directed mutagenesis of the conserved cysteines (C68, C72) and histidines (H81, H85) present within this motif to alanine. The results indicate that the zinc finger motif is indeed involved in binding to zinc and DNA. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

A conserved zinc finger motif in the coat protein of Tomato leaf curl Bangalore virus is responsible for binding to ssDNA

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Publisher
Springer-Verlag
Copyright
Copyright © 2003 by Springer-Verlag/Wien
Subject
LifeSciences
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-003-0174-x
Publisher site
See Article on Publisher Site

Abstract

Tomato leaf curl Bangalore virus (ToLCBV-(Ban5)) from south India belongs to the genus Begomovirus of the family Geminiviridae . So far, only the DNA A component has been identified in this virus. In this paper we report the overexpression of ToLCBV-(Ban5) coat protein (CP) in E. coli and its characterization. The purified recombinant CP bound preferentially to ssDNA in a sequence non-specific manner. Deletion of 50 amino acids from the N-terminus, including a putative N-terminal α helix, did not result in the loss of binding to DNA. A search for motifs responsible for DNA binding indicated a conserved putative zinc finger motif in the CPs (corresponding to residues 65–85 in ToLCBV-(Ban 5) CP) of begomoviruses. The role of this motif in DNA binding was evaluated through site-directed mutagenesis of the conserved cysteines (C68, C72) and histidines (H81, H85) present within this motif to alanine. The results indicate that the zinc finger motif is indeed involved in binding to zinc and DNA.

Journal

Archives of VirologySpringer Journals

Published: Dec 1, 2003

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