A comparative analysis of the Far Eastern grayling species Thymallus tugarinae and Thymallus grubii flavomaculatus based on the data from mitochondrial DNA cytochrome b gene sequencing

A comparative analysis of the Far Eastern grayling species Thymallus tugarinae and Thymallus... Nucleotide sequences of a fragment of mitochondrial DNA cytochrome b gene were obtained from two species of graylings that inhabit the Russian Far East, viz., Thymallus tugarinae and T. grubii flavomaculatus. A phylogenetic analysis of four Thymallus species, whose relationships have been poorly studied, was performed on these new data as well as on several sequences from the NCBI GenBank sequence database. The rate of genetic divergence between the Far Eastern graylings Thymallus tugarinae and T. g. flavomaculatus corresponded to that between European (T. thymallus) and Arctic (T. arcticus) graylings. Moreover, single nucleotide substitutions that result in alterations of the amino-acid structure of protein products (non-synonymic mutations) were revealed between sequences of the cytochrome b gene in the Far Eastern grayling species. The topology of phylogenetic trees, which was composed by means of Bayesian analysis and the maximum parsimony method, showed four independent phylogenetic lineages of graylings. In addition, the phylogenetic relationships between T. tugarinae and T. g. flavomaculatus were supported statistically. The obtained data indicated that Far Eastern graylings T. tugarinae and T. g. flavomaculatus are distinct species and confirmed that they belong to different phylogenetic lineages, as found earlier. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Marine Biology Springer Journals

A comparative analysis of the Far Eastern grayling species Thymallus tugarinae and Thymallus grubii flavomaculatus based on the data from mitochondrial DNA cytochrome b gene sequencing

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Publisher
Springer Journals
Copyright
Copyright © 2012 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Freshwater & Marine Ecology
ISSN
1063-0740
eISSN
1608-3377
D.O.I.
10.1134/S1063074012070024
Publisher site
See Article on Publisher Site

Abstract

Nucleotide sequences of a fragment of mitochondrial DNA cytochrome b gene were obtained from two species of graylings that inhabit the Russian Far East, viz., Thymallus tugarinae and T. grubii flavomaculatus. A phylogenetic analysis of four Thymallus species, whose relationships have been poorly studied, was performed on these new data as well as on several sequences from the NCBI GenBank sequence database. The rate of genetic divergence between the Far Eastern graylings Thymallus tugarinae and T. g. flavomaculatus corresponded to that between European (T. thymallus) and Arctic (T. arcticus) graylings. Moreover, single nucleotide substitutions that result in alterations of the amino-acid structure of protein products (non-synonymic mutations) were revealed between sequences of the cytochrome b gene in the Far Eastern grayling species. The topology of phylogenetic trees, which was composed by means of Bayesian analysis and the maximum parsimony method, showed four independent phylogenetic lineages of graylings. In addition, the phylogenetic relationships between T. tugarinae and T. g. flavomaculatus were supported statistically. The obtained data indicated that Far Eastern graylings T. tugarinae and T. g. flavomaculatus are distinct species and confirmed that they belong to different phylogenetic lineages, as found earlier.

Journal

Russian Journal of Marine Biology – Springer Journals

Published: Jan 4, 2013

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