A comparative analysis of variability of heterologous gene expression was conducted in original (T0) transgenic tobacco plants, differing with respect to the copy number of uidA gene (a single and two copies as a direct duplication) within the T-DNA insertion, and among progenies obtained by their self-pollination (T1). Variability of β-glucuronidase enzyme activity was shown in original transgenic plants or among progenies of the first generation in both groups. It is established that the activity level of β-glucuronidase among T0 plants with a duplication of the uidA gene is significantly higher (21.9 times) than that determined in transformants with a single-copy gene. In T1 progenies of plant 16.40, carrying a duplication of the uidA gene, a drastic decrease in enzyme activity was observed, which correlated with a decrease or absence of mRNA transcripts, pointing to a disturbance in reporter gene expression. Alterations in the functioning of another heterologous gene (nptII) within the same T-DNA insertion were not observed among progenies of transgenic plant 16.40.
Russian Journal of Genetics – Springer Journals
Published: May 17, 2006
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