A change in the stability of marker nptII and uidA gene expression in transgenic tobacco plants

A change in the stability of marker nptII and uidA gene expression in transgenic tobacco plants A comparative analysis of variability of heterologous gene expression was conducted in original (T0) transgenic tobacco plants, differing with respect to the copy number of uidA gene (a single and two copies as a direct duplication) within the T-DNA insertion, and among progenies obtained by their self-pollination (T1). Variability of β-glucuronidase enzyme activity was shown in original transgenic plants or among progenies of the first generation in both groups. It is established that the activity level of β-glucuronidase among T0 plants with a duplication of the uidA gene is significantly higher (21.9 times) than that determined in transformants with a single-copy gene. In T1 progenies of plant 16.40, carrying a duplication of the uidA gene, a drastic decrease in enzyme activity was observed, which correlated with a decrease or absence of mRNA transcripts, pointing to a disturbance in reporter gene expression. Alterations in the functioning of another heterologous gene (nptII) within the same T-DNA insertion were not observed among progenies of transgenic plant 16.40. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

A change in the stability of marker nptII and uidA gene expression in transgenic tobacco plants

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Publisher
Springer Journals
Copyright
Copyright © 2006 by Pleiades Publishing, Inc.
Subject
Biomedicine; Microbial Genetics and Genomics; Animal Genetics and Genomics; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795406050085
Publisher site
See Article on Publisher Site

Abstract

A comparative analysis of variability of heterologous gene expression was conducted in original (T0) transgenic tobacco plants, differing with respect to the copy number of uidA gene (a single and two copies as a direct duplication) within the T-DNA insertion, and among progenies obtained by their self-pollination (T1). Variability of β-glucuronidase enzyme activity was shown in original transgenic plants or among progenies of the first generation in both groups. It is established that the activity level of β-glucuronidase among T0 plants with a duplication of the uidA gene is significantly higher (21.9 times) than that determined in transformants with a single-copy gene. In T1 progenies of plant 16.40, carrying a duplication of the uidA gene, a drastic decrease in enzyme activity was observed, which correlated with a decrease or absence of mRNA transcripts, pointing to a disturbance in reporter gene expression. Alterations in the functioning of another heterologous gene (nptII) within the same T-DNA insertion were not observed among progenies of transgenic plant 16.40.

Journal

Russian Journal of GeneticsSpringer Journals

Published: May 17, 2006

References

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