A C. elegans Model for the Study of RAGE-Related Neurodegeneration

A C. elegans Model for the Study of RAGE-Related Neurodegeneration The receptor for advanced glycation products (RAGE) is a cell surface, multi-ligand receptor belonging to the immu- noglobulin superfamily; this receptor is implicated in a variety of maladies, via inflammatory pathways and induction of oxidative stress. Currently, RAGE is being studied using a limited number of mammalian in vivo, and some comple- mentaryinvitro,models.Here,we present a Caenorhabditis elegans model for the study of RAGE-related pathology: a transgenic strain, expressing RAGE in all neurons, was generated and subsequently tested behaviorally, developmentally, and morphologically. In addition to RAGE expression being associated with a significantly shorter lifespan, the follow- ing behavioral observations were made when RAGE-expressing worms were compared to the wild type: RAGE- expressing wormsshowedanimpaireddopaminergic system, evaluated by measuring the fluorescent signal of GFP tagging; these worms exhibited decreased locomotion—both general and following ethanol exposure—as measured by counting body bends in adult worms; RAGE expression was also associated with impaired recovery of quiescence and pharyngeal pumping secondary to heat shock, as a significantly smaller fraction of RAGE-expressing worms engaged in these behaviors in the 2 h immediately following the heat shock. Finally, significant developmental differences were also found between the two strains: RAGE expression leads to a significantly smaller fraction of hatched http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Neurotoxicity Research Springer Journals

A C. elegans Model for the Study of RAGE-Related Neurodegeneration

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Publisher
Springer US
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Biomedicine; Neurosciences; Neurology; Neurochemistry; Pharmacology/Toxicology; Neurobiology; Cell Biology
ISSN
1029-8428
eISSN
1476-3524
D.O.I.
10.1007/s12640-018-9918-y
Publisher site
See Article on Publisher Site

Abstract

The receptor for advanced glycation products (RAGE) is a cell surface, multi-ligand receptor belonging to the immu- noglobulin superfamily; this receptor is implicated in a variety of maladies, via inflammatory pathways and induction of oxidative stress. Currently, RAGE is being studied using a limited number of mammalian in vivo, and some comple- mentaryinvitro,models.Here,we present a Caenorhabditis elegans model for the study of RAGE-related pathology: a transgenic strain, expressing RAGE in all neurons, was generated and subsequently tested behaviorally, developmentally, and morphologically. In addition to RAGE expression being associated with a significantly shorter lifespan, the follow- ing behavioral observations were made when RAGE-expressing worms were compared to the wild type: RAGE- expressing wormsshowedanimpaireddopaminergic system, evaluated by measuring the fluorescent signal of GFP tagging; these worms exhibited decreased locomotion—both general and following ethanol exposure—as measured by counting body bends in adult worms; RAGE expression was also associated with impaired recovery of quiescence and pharyngeal pumping secondary to heat shock, as a significantly smaller fraction of RAGE-expressing worms engaged in these behaviors in the 2 h immediately following the heat shock. Finally, significant developmental differences were also found between the two strains: RAGE expression leads to a significantly smaller fraction of hatched

Journal

Neurotoxicity ResearchSpringer Journals

Published: Jun 4, 2018

References

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