A broadly reactive monoclonal antibody detects multiple genotypes of hepatitis B virus X protein

A broadly reactive monoclonal antibody detects multiple genotypes of hepatitis B virus X protein A highly specific and broadly reactive monoclonal antibody against hepatitis B virus X (HBx) protein was developed that detected, in both immunoblotting and immunofluorescence, HBx proteins of seven of the eight currently known genotypes of HBV, which were overexpressed in cultured cells. Evaluation of HBx expression levels in cultured hepatocytes using this monoclonal antibody showed that cells transiently and stably transfected with HBV genomes expressed far less HBx protein than cells transiently transfected with an HBx overexpression plasmid routinely used for studying HBx functions. The availability of such sensitive and broadly reactive monoclonal antibodies against HBx will enable more-quantitative studies of HBx functions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

A broadly reactive monoclonal antibody detects multiple genotypes of hepatitis B virus X protein

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Publisher
Springer Journals
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2111-6
Publisher site
See Article on Publisher Site

Abstract

A highly specific and broadly reactive monoclonal antibody against hepatitis B virus X (HBx) protein was developed that detected, in both immunoblotting and immunofluorescence, HBx proteins of seven of the eight currently known genotypes of HBV, which were overexpressed in cultured cells. Evaluation of HBx expression levels in cultured hepatocytes using this monoclonal antibody showed that cells transiently and stably transfected with HBV genomes expressed far less HBx protein than cells transiently transfected with an HBx overexpression plasmid routinely used for studying HBx functions. The availability of such sensitive and broadly reactive monoclonal antibodies against HBx will enable more-quantitative studies of HBx functions.

Journal

Archives of VirologySpringer Journals

Published: Oct 1, 2014

References

  • Hepatitis B virus genotype G forms core-like particles with unique structural properties
    Cotelesage, JJ; Osiowy, C; Lawrence, C; DeVarennes, SL; Teow, S; Beniac, DR; Booth, TF
  • Hepatitis B virus X protein: a multifunctional viral regulator
    Murakami, S
  • Worldwide genetic diversity of HBV genotypes and risk of hepatocellular carcinoma
    Pujol, FH; Navas, MC; Hainaut, P; Chemin, I
  • Hepatitis B virus: significance of genotypes
    Schaefer, S

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