A broadly reactive monoclonal antibody detects multiple genotypes of hepatitis B virus X protein

A broadly reactive monoclonal antibody detects multiple genotypes of hepatitis B virus X protein A highly specific and broadly reactive monoclonal antibody against hepatitis B virus X (HBx) protein was developed that detected, in both immunoblotting and immunofluorescence, HBx proteins of seven of the eight currently known genotypes of HBV, which were overexpressed in cultured cells. Evaluation of HBx expression levels in cultured hepatocytes using this monoclonal antibody showed that cells transiently and stably transfected with HBV genomes expressed far less HBx protein than cells transiently transfected with an HBx overexpression plasmid routinely used for studying HBx functions. The availability of such sensitive and broadly reactive monoclonal antibodies against HBx will enable more-quantitative studies of HBx functions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

A broadly reactive monoclonal antibody detects multiple genotypes of hepatitis B virus X protein

Loading next page...
Springer Vienna
Copyright © 2014 by Springer-Verlag Wien
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
Publisher site
See Article on Publisher Site


  • Hepatitis B virus genotype G forms core-like particles with unique structural properties
    Cotelesage, JJ; Osiowy, C; Lawrence, C; DeVarennes, SL; Teow, S; Beniac, DR; Booth, TF
  • Hepatitis B virus X protein: a multifunctional viral regulator
    Murakami, S
  • Worldwide genetic diversity of HBV genotypes and risk of hepatocellular carcinoma
    Pujol, FH; Navas, MC; Hainaut, P; Chemin, I
  • Hepatitis B virus: significance of genotypes
    Schaefer, S

You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial