Mammalian Genome 10, 837–838 (1999). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1999 1 2 3 3 4 4 David Hills, Simon Tracey, Julio Masabanda, Ruedi Fries, Leonard C. Schalkwyk, Hans Lehrach, 2 1 J. Ross Miller, John L. Williams Roslin Institute, Roslin, Midlothian, EH25 9PS, UK Babraham Institute, Babraham, Cambridge, CB2 4AT, UK Technical University of Munich, D-85350 Freising-Weihenstephan, Germany Max Planck Institute, D-14195 Berlin, Germany Received: 29 January 1999 / Accepted: 16 April 1999 Successful positional-cloning strategies in human and mouse have (typically 1:200 u) as described by Larin and coworkers (1991), relied upon the wide availability of numerous large-fragment li- then size selected by electrophoresis in a 1% Sea Plaque agarose braries (Collins 1995). Although genomic analysis of livestock gel with a Bio-Rad CHEF pulsed field gel apparatus (set at 30 s species is not as advanced as that in human and mouse, it is switch time, 4.7 v/cm at 14°C in 0.5 × TBE for 18 h). The agarose progressing rapidly. In cattle there are now over 746 loci assigned slice containing the unresolved compression zone (>400 kb in to the genetic linkage map, with an average marker interval of these conditions) was recovered
Mammalian Genome – Springer Journals
Published: Aug 1, 1999
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