Plant Mol Biol (2017) 93:269–281 DOI 10.1007/s11103-016-0560-x A bifunctional aminoglycoside acetyltransferase/ phosphotransferase conferring tobramycin resistance provides an efficient selectable marker for plastid transformation 1 1 1 Iman Tabatabaei · Stephanie Ruf · Ralph Bock Received: 30 September 2016 / Accepted: 10 November 2016 / Published online: 17 November 2016 © The Author(s) 2016. This article is published with open access at Springerlink.com Abstract aminoglycoside antibiotics highly efficiently. We report Key message A new selectable marker gene for stable that, in combination with selection for resistance to the transformation of the plastid genome was developed aminoglycoside tobramycin, the aac(6′)-Ie/aph(2″)-Ia gene that is similarly efficient as the aadA, and produces no represents an efficient marker for plastid transformation in background of spontaneous resistance mutants. that it produces similar numbers of transplastomic lines Abstract More than 25 years after its development for as the spectinomycin resistance gene aadA. Importantly, Chlamydomonas and tobacco, the transformation of the no spontaneous antibiotic resistance mutants appear under chloroplast genome still represents a challenging technol- tobramycin selection. ogy that is available only in a handful of species. The vast majority of chloroplast transformation experiments con- Keywords Plastid transformation · Nicotiana tabacum · ducted thus far have relied on a single selectable marker Selectable
Plant Molecular Biology – Springer Journals
Published: Nov 17, 2016
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