5′ deletion of a gbss1 promoter region from wheat leads to changes in tissue and developmental specificities

5′ deletion of a gbss1 promoter region from wheat leads to changes in tissue and developmental... Expression of granule-bound starch synthase 1 (GBSSI) in wheat is restricted to the grain filling process. In order to identify promoter regions which are involved in transcriptional control of the observed expression pattern, we isolated about 8 kb of a wheat gbss1-upstream region. Within this sequence several putative cis-acting elements were identified. In addition, an untranslated leader region is located in the 5′ region of the gbss1gene. To investigate promoter activity of the isolated region, the proximal 4.0 kb and progressively 5′-deleted fragments were transcriptionally fused to a β-glucuronidase reporter gene. The function of the promoter constructs was tested by transient expression assays in various wheat tissues and in transgenic wheat plants, which were selected for low number and integrity of transgene copies. Analysis of stable transformants revealed that the −4.0 kb promoter region mediates reporter gene expression that is in accordance with the endogenous gbss1expression. Promoter deletion to −1.9 kb or to −1.0 kb did not change the expression profile with regard to grain and pollen specificity. However, the profile of β-glucuronidase expression during the grain filling process is altered in such a way that the level of β-glucuronidase activity declines due to the decreasing promoter length. It is proposed that enhancer elements and cis-acting elements, which are involved in gbss1transcription during the grain filling process, are located −1.9 kb upstream of the promoter. In addition, participation of the untranslated leader region in tissue-specific gene expression is discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

5′ deletion of a gbss1 promoter region from wheat leads to changes in tissue and developmental specificities

Loading next page...
 
/lp/springer_journal/5-deletion-of-a-gbss1-promoter-region-from-wheat-leads-to-changes-in-wGHR9jEBSU
Publisher
Springer Journals
Copyright
Copyright © 2002 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1015576930688
Publisher site
See Article on Publisher Site

Abstract

Expression of granule-bound starch synthase 1 (GBSSI) in wheat is restricted to the grain filling process. In order to identify promoter regions which are involved in transcriptional control of the observed expression pattern, we isolated about 8 kb of a wheat gbss1-upstream region. Within this sequence several putative cis-acting elements were identified. In addition, an untranslated leader region is located in the 5′ region of the gbss1gene. To investigate promoter activity of the isolated region, the proximal 4.0 kb and progressively 5′-deleted fragments were transcriptionally fused to a β-glucuronidase reporter gene. The function of the promoter constructs was tested by transient expression assays in various wheat tissues and in transgenic wheat plants, which were selected for low number and integrity of transgene copies. Analysis of stable transformants revealed that the −4.0 kb promoter region mediates reporter gene expression that is in accordance with the endogenous gbss1expression. Promoter deletion to −1.9 kb or to −1.0 kb did not change the expression profile with regard to grain and pollen specificity. However, the profile of β-glucuronidase expression during the grain filling process is altered in such a way that the level of β-glucuronidase activity declines due to the decreasing promoter length. It is proposed that enhancer elements and cis-acting elements, which are involved in gbss1transcription during the grain filling process, are located −1.9 kb upstream of the promoter. In addition, participation of the untranslated leader region in tissue-specific gene expression is discussed.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 13, 2004

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off