Immunohistochemistry Using Polyester Wax
AbstractFor the preparation of sections for immunohistochemistry at the level of the light microscope, the choice of sectioning medium usually lies between frozen (fresh or fixed-cryoprotected) or paraffin wax. If the antigen survives fixation and processing at 60°C, paraffin wax is used. If the antigen survives fixation, but not heating to 60°C, frozen sections from fixed cryoprotected tissue are prepared, but if the antigen does not survive chemical fixation, sections are prepared from fresh, rapid-frozen tissue, and a trade-off in morphological preservation is accepted. However, if the antigen survives chemical fixation, but is sensitive to temperatures above 40°C (as many are to some degree) or is soluble in the clearing agent, polyester wax offers an opportunity for immunostaining without having to resort to frozen sections.