Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules

Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale... Density gradient ultracentrifugation has been widely used to fractionate animal, plant, and bacterial cells, viral particles, lysosomes, membranes, and mac-romolecules in a range of processes. Its application has been of particular significance in the commercial preparation of viruses for vaccine and immuno-therapy products in both batch and continuous-flow zonal modes. These methods have been traditionally used to purify influenza vaccines ( 1 ), but more recently have been used in devising new vaccine purifications such as Hepatitis B ( 2 ) and Rabies ( 3 ). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules

Desai, Mohamed A.; Merino, Sandra P.
Downstream Processing of Proteins, Volume 9: 6
Springer Protocols – Jan 1, 2000
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Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules

Abstract

Density gradient ultracentrifugation has been widely used to fractionate animal, plant, and bacterial cells, viral particles, lysosomes, membranes, and mac-romolecules in a range of processes. Its application has been of particular significance in the commercial preparation of viruses for vaccine and immuno-therapy products in both batch and continuous-flow zonal modes. These methods have been traditionally used to purify influenza vaccines ( 1 ), but more recently have been used in devising new vaccine purifications such as Hepatitis B ( 2 ) and Rabies ( 3 ).
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Downstream Processing of Proteins, Volume 9: 6
Desai, Mohamed A.; Merino, Sandra P.
Springer Protocols – Jan 1, 2000
Downstream Processing of Proteins, Volume 9: 6
Desai, Mohamed A.; Merino, Sandra P.
Springer Protocols – Jan 1, 2000
Publisher site
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Abstract

Density gradient ultracentrifugation has been widely used to fractionate animal, plant, and bacterial cells, viral particles, lysosomes, membranes, and mac-romolecules in a range of processes. Its application has been of particular significance in the commercial preparation of viruses for vaccine and immuno-therapy products in both batch and continuous-flow zonal modes. These methods have been traditionally used to purify influenza vaccines ( 1 ), but more recently have been used in devising new vaccine purifications such as Hepatitis B ( 2 ) and Rabies ( 3 ).

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