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What hPSCs need

What hPSCs need research highlights steM CeLLs IN BRIEF GeNoMICs Yilmaz, A. et al. Nat. Cell Biol. 20, 610–619 (2018). a pan-cancer atlas Hoadley, K. A. et al. Cell 173, 291–304 (2018). To discover how much something is needed, it is a good strategy to try going The Cancer Genome Atlas (TCGA), a without it. Yilmaz et al. applied this program funded by the US National Institutes approach to characterize genes essential of Health (NIH), has ended over a decade for the maintenance of human pluripotent after its inception. The ambitious project stem cells (PSCs). They combined two coordinated the multi-omic sequencing and previously characterized tools: a haploid clinical annotation of approximately 10,000 human PSC line and CRISPR-based tumors across 33 cancer types. The resulting knockout screening. Along with Cas9, the resource, publicly available through the researchers delivered a library of single guide NIH Genomic Data Commons (https://gdc. RNAs (sgRNAs) targeting 18,000 genes with cancer.gov/), includes data on DNA sequence, 10 sgRNAs per gene to the cells and assessed DNA methylation, RNA and microRNA cell survival and growth rate, as well as the expression, and reverse-phase protein array abundance of sgRNAs over time. This study binding, and makes it possible to rigorously http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Methods Springer Journals

What hPSCs need

Rusk, Nicole
Nature Methods , Volume 15 (6) – May 31, 2018
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What hPSCs need

Abstract

research highlights steM CeLLs IN BRIEF GeNoMICs Yilmaz, A. et al. Nat. Cell Biol. 20, 610–619 (2018). a pan-cancer atlas Hoadley, K. A. et al. Cell 173, 291–304 (2018). To discover how much something is needed, it is a good strategy to try going The Cancer Genome Atlas (TCGA), a without it. Yilmaz et al. applied this program funded by the US National Institutes approach to characterize genes essential of Health (NIH), has ended over a decade for the maintenance of human...
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Publisher
Springer Journals
Copyright
Copyright © 2018 by The Author(s)
Subject
Life Sciences; Life Sciences, general; Biological Techniques; Biological Microscopy; Biomedical Engineering/Biotechnology; Bioinformatics; Proteomics
ISSN
1548-7091
eISSN
1548-7105
DOI
10.1038/s41592-018-0022-2
Publisher site
See Article on Publisher Site

Abstract

research highlights steM CeLLs IN BRIEF GeNoMICs Yilmaz, A. et al. Nat. Cell Biol. 20, 610–619 (2018). a pan-cancer atlas Hoadley, K. A. et al. Cell 173, 291–304 (2018). To discover how much something is needed, it is a good strategy to try going The Cancer Genome Atlas (TCGA), a without it. Yilmaz et al. applied this program funded by the US National Institutes approach to characterize genes essential of Health (NIH), has ended over a decade for the maintenance of human pluripotent after its inception. The ambitious project stem cells (PSCs). They combined two coordinated the multi-omic sequencing and previously characterized tools: a haploid clinical annotation of approximately 10,000 human PSC line and CRISPR-based tumors across 33 cancer types. The resulting knockout screening. Along with Cas9, the resource, publicly available through the researchers delivered a library of single guide NIH Genomic Data Commons (https://gdc. RNAs (sgRNAs) targeting 18,000 genes with cancer.gov/), includes data on DNA sequence, 10 sgRNAs per gene to the cells and assessed DNA methylation, RNA and microRNA cell survival and growth rate, as well as the expression, and reverse-phase protein array abundance of sgRNAs over time. This study binding, and makes it possible to rigorously

Journal

Nature Methods – Springer Journals

Published: May 31, 2018

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