Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Untersuchungen zur Isolierung, Stoffwechselphysiologie und Morphologie von Gallionella ferruginea Ehrenberg

Untersuchungen zur Isolierung, Stoffwechselphysiologie und Morphologie von Gallionella ferruginea... 203 60 60 4 4 Helmut Hanert Mikrobiologische Abteilung des Staatsinstituts für Allgemeine Botanik und Botanischer Garten Hamburg Hamburg Deutschland Botanisches Institut der Technischen Hochschule Humboldtstraße 1 3300 Braunschweig Summary 1. Gallionella ferruginea has been isolated from enrichment cultures contaminated by pseudomonads. 2. Pure cultures do not contain any pseudomonads; inoculated yeast-water bouillon does not get turbid. 3. Gallionella -colonies in a diameter of 1.2 mm are composed of about 60,000 to 80,000 generative units. 4. Growth of Gallionella is encouraged by inoculating pseudomonads ( Ps. fluorescens ) to the pure cultures probably owing to a decrease of O 2 -content. 5. Growth is increased by low O 2 -concentrations (0.1–0.2 mg O 2 /l) dissolved in culture medium; higher O 2 -contents (above 2.75 mg O 2 /l) are inhibitting. 6. Further physiological studies on Fe(II)-autoxidation and redoxpotentials proved that both are quite more intensive in 5 sec CO 2 -bubbled growth-reducing FeS-mediums than in microaerophilic growth-encouraging ones. 7. In electron microscopic investigations no terminal cells have been observed in pure cultures. Gallionella -threads however showed segmented filament-structures, which seem to support the assumption that these segments (diameter <=0.1 μ) are microcellulare reproduction-units. Probable stages of development have been observed twice. 8. Gallionella -threads absorb in about the same UV-sphere as nucleic acids and proteins. 9. Gallionella -colonies growing in NaH 14 CO 3 -FeS-medium showed a measurable fixation of 14 CO 2 . http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

Untersuchungen zur Isolierung, Stoffwechselphysiologie und Morphologie von Gallionella ferruginea Ehrenberg

Archives of Microbiology , Volume 60 (4) – Dec 1, 1968

Loading next page...
 
/lp/springer-journals/untersuchungen-zur-isolierung-stoffwechselphysiologie-und-morphologie-AURlPa5Ghd

References (21)

Publisher
Springer Journals
Copyright
Copyright © 1968 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Biochemistry, general; Cell Biology; Ecology; Microbial Ecology; Microbiology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/BF00408555
Publisher site
See Article on Publisher Site

Abstract

203 60 60 4 4 Helmut Hanert Mikrobiologische Abteilung des Staatsinstituts für Allgemeine Botanik und Botanischer Garten Hamburg Hamburg Deutschland Botanisches Institut der Technischen Hochschule Humboldtstraße 1 3300 Braunschweig Summary 1. Gallionella ferruginea has been isolated from enrichment cultures contaminated by pseudomonads. 2. Pure cultures do not contain any pseudomonads; inoculated yeast-water bouillon does not get turbid. 3. Gallionella -colonies in a diameter of 1.2 mm are composed of about 60,000 to 80,000 generative units. 4. Growth of Gallionella is encouraged by inoculating pseudomonads ( Ps. fluorescens ) to the pure cultures probably owing to a decrease of O 2 -content. 5. Growth is increased by low O 2 -concentrations (0.1–0.2 mg O 2 /l) dissolved in culture medium; higher O 2 -contents (above 2.75 mg O 2 /l) are inhibitting. 6. Further physiological studies on Fe(II)-autoxidation and redoxpotentials proved that both are quite more intensive in 5 sec CO 2 -bubbled growth-reducing FeS-mediums than in microaerophilic growth-encouraging ones. 7. In electron microscopic investigations no terminal cells have been observed in pure cultures. Gallionella -threads however showed segmented filament-structures, which seem to support the assumption that these segments (diameter <=0.1 μ) are microcellulare reproduction-units. Probable stages of development have been observed twice. 8. Gallionella -threads absorb in about the same UV-sphere as nucleic acids and proteins. 9. Gallionella -colonies growing in NaH 14 CO 3 -FeS-medium showed a measurable fixation of 14 CO 2 .

Journal

Archives of MicrobiologySpringer Journals

Published: Dec 1, 1968

There are no references for this article.