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E. Hood, S. Gelvin, L. Melchers, A. Hoekema (1993)
NewAgrobacterium helper plasmids for gene transfer to plantsTransgenic Research, 2
D. Pelah, O. Shoseyov, A. Altman (1995)
Characterization of BspA, a major boiling-stable, water-stress-responsive protein in aspen (Populus tremula).Tree physiology, 15 10
A. Guivarc’h, J. Caissard, A. Azmi, T. Elmayan, D. Chriqui, M. Tepfer (1996)
In situ detection of expression of thegus reporter gene in transgenic plants: ten years of blue genesTransgenic Research, 5
G. Lazo, P. Stein, R. Ludwig (1991)
A DNA Transformation–Competent Arabidopsis Genomic Library in AgrobacteriumBio/Technology, 9
Marc Goldstein, Masahiro Takagi, Seiichi Hashida, O. Shoseyov, Roy Doi, I. Segel (1993)
Characterization of the cellulose-binding domain of the Clostridium cellulovorans cellulose-binding protein AJournal of Bacteriology, 175
Elizabeth Hood, G. Helmer, Robert Fraley, M. Chilton (1986)
The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNAJournal of Bacteriology, 168
B. Janssen, R. Gardner (2004)
Localized transient expression of GUS in leaf discs following cocultivation with AgrobacteriumPlant Molecular Biology, 14
S. Broido, A. Loyter, A. Vainstein (1993)
Transient expression of photosynthetic genes in transfected albinoid petunia protoplasts and correct processing of newly synthesized chloroplast-destined polypeptidesPhysiologia Plantarum, 88
K.H. Han, M.P. Gordon, S.H. Strauss (1996)
Biology of Populus and its Implications for Management and Conservation
J. Haas, L. Moore, W. Ream, S. Manulis (1995)
Universal PCR primers for detection of phytopathogenic Agrobacterium strainsApplied and Environmental Microbiology, 61
T. Tzfira, C. Jensen, A. Vainstein, A. Altman (1997)
Transformation and regeneration of transgenic aspen plants via shoot formation from stem explantsPhysiologia Plantarum, 99
P. Bret (1993)
Le dernier des procédés révolutionnaires: la fabrication et l'expertise de la poudre ronde (1795-1830)Annals of Science, 50
(1992)
Histochemical detection of GUS, pp
K. Mcbride, K. Summerfelt (1990)
Improved binary vectors for Agrobacterium-mediated plant transformationPlant Molecular Biology, 14
Thomas Schmülling, Jozef Schell, Angelo Spena (1988)
Single genes from Agrobacterium rhizogenes influence plant developmentThe EMBO Journal, 7
(1986)
Aspen, pp
J.C. Sellmer, B.H. McCown (1989)
Transformation in Populus spp, pp. in Plant Protoplasts and Genetic Engineering II
(1996)
Cellular and molecular biology of Agrobacterium-mediated transformation of plants and its application to genetic transformation of Populus, pp
L. Jouanin, A. Brasileiro, J. Leplé, G. Pilate, D. Cornu (1993)
Genetic transformation: a short review of methods and their applications, results and perspectives for forest treesAnnals of Forest Science, 50
D. Becker (1990)
Binary vectors which allow the exchange of plant selectable markers and reporter genesNucleic acids research, 18 1
T. Murashige, F. Skoog (1962)
A revised medium for rapid growth and bio assays with tobacco tissue culturesPhysiologia Plantarum, 15
D. Pelah, O. Shoseyov, A. Altman (1996)
Characterization of BspA, a major accumulation of water–stress–responsive protien in aspen (Populus tremula)Tree Physiol., 15
J. Sellmer, B. Mccown (1989)
Transformation in Populus spp.
M. Bernaerts, J. Ley (1963)
A Biochemical Test for Crown Gall BacteriaNature, 197
Hong Wang, Meiqing Qi, A. Cutler (1993)
A simple method of preparing plant samples for PCR.Nucleic acids research, 21 17
(1995)
Cloning and characterization of endo1,4- -glucanase in Arabidopsis thaliana
G. Lloyd, B. Mccown (1980)
Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture, 30
A.M. Stomp (1992)
GUS Protocols: Using the GUS Gene as a Reporter of Gene Expression
In recent years, Populus species have acquired an important place in basic and applied research of woody plants. The practical role of Populus species in world forestry and their importance to research as a woody-plant model have led to increasing interest in tissue-culture and molecular techniques, as well as the development of transformation procedures for this genus. A simple technical procedure is described here step-by-step, for the first time, as a routine method for transforming Populus tremula using a disarmed Agrobacterium tumefaciens hypervirulent strain. The procedure begins with the inoculation of stem explants with bacterial suspension, followed by a short period of co-cultivation on a highly regenerative medium. Transformed shoots are selected on regeneration medium containing antibiotics and the presence of the inserted target genes is checked using a rapid and efficient PCR test. Selected shoots are transferred to a rooting medium, under the same selection pressure, and propagated via stem cuttings. Selected plants can be hardened and transferred to the green-house within 4 months of inoculation. The method has proven efficient for several gene constructs, selection on Kan or Hyg, and three different Agrobacterium strains.
Plant Molecular Biology Reporter – Springer Journals
Published: Sep 29, 2004
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