Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Towards the Genotyping of Fungi: Methods, Benefits and Challenges

Towards the Genotyping of Fungi: Methods, Benefits and Challenges Several fungi have recently been described as capable to recombine and drive large genetic diversity in clinical samples and in the environment. Among the genotyping methods, microsatellite analysis is frequently reported as preferred for studying local epidemiology, but single nucleotide polymorphisms represent the best markers for evaluation of recombination, linkage and aneuploidy. The future of typing analyses may reside in strategies capable of cataloging the whole genome and complete microbial diversity. The present review focuses the current strategies employed for fungal genotyping and evaluation of genetic diversity, and the challenges of next generation sequencing with regard to this topic. Typing methods establish the genetic identity of fungal isolates and allow clarification of outbreaks and transmission of strains between individuals, comparison of chronic colonization versus patients carrying unrelated strains, detection of co-evolution of pathogenic and/or drug-resistant strains. The next advances in molecular mycology may revolutionize clinics and redesign concepts of microbial evolution. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Fungal Infection Reports Springer Journals

Towards the Genotyping of Fungi: Methods, Benefits and Challenges

Current Fungal Infection Reports , Volume 8 (3) – Jun 13, 2014

Loading next page...
 
/lp/springer-journals/towards-the-genotyping-of-fungi-methods-benefits-and-challenges-iayCqEwRW9

References (84)

Publisher
Springer Journals
Copyright
Copyright © 2014 by Springer Science+Business Media New York
Subject
Medicine & Public Health; Infectious Diseases; Internal Medicine; Tropical Medicine; Occupational Medicine/Industrial Medicine; Pneumology/Respiratory System
ISSN
1936-3761
eISSN
1936-377X
DOI
10.1007/s12281-014-0190-1
Publisher site
See Article on Publisher Site

Abstract

Several fungi have recently been described as capable to recombine and drive large genetic diversity in clinical samples and in the environment. Among the genotyping methods, microsatellite analysis is frequently reported as preferred for studying local epidemiology, but single nucleotide polymorphisms represent the best markers for evaluation of recombination, linkage and aneuploidy. The future of typing analyses may reside in strategies capable of cataloging the whole genome and complete microbial diversity. The present review focuses the current strategies employed for fungal genotyping and evaluation of genetic diversity, and the challenges of next generation sequencing with regard to this topic. Typing methods establish the genetic identity of fungal isolates and allow clarification of outbreaks and transmission of strains between individuals, comparison of chronic colonization versus patients carrying unrelated strains, detection of co-evolution of pathogenic and/or drug-resistant strains. The next advances in molecular mycology may revolutionize clinics and redesign concepts of microbial evolution.

Journal

Current Fungal Infection ReportsSpringer Journals

Published: Jun 13, 2014

There are no references for this article.