The mode of myofibril remodelling in human skeletal muscle affected by DOMS induced by eccentric contractions

The mode of myofibril remodelling in human skeletal muscle affected by DOMS induced by eccentric... Ji-Guo Yu Dieter O. Fürst Lars-Eric Thornell +46-90-7865142 +46-90-7865480 lars-eric.thornell@anatomy.umu.se Department of Integrative Medical Biology, Section for Anatomy, Umeå University, 901 87, Umeå, Sweden Department of Musculoskeletal Research, Gävle University, 907 13, Gävle, Sweden Department of Cell Biology, Institute for Biochemistry and Biology, University of Potsdam, Potsdam, Germany Myofibrillar Z-disc streaming and loss of the desmin cytoskeleton are considered the morphological hallmarks of eccentric contraction-induced injury. The latter is contradicted by recent studies where a focal increase of desmin was observed in biopsies taken from human muscles with DOMS. In order to determine the effects of eccentric contraction-induced alterations of the myofibrillar Z-disc, we examined the distribution of α-actinin, the Z-disc portion of titin and the nebulin NB2 region in relation to actin and desmin in DOMS biopsies. In biopsies taken 2–3 days and 7–8 days after exercise, we observed a significantly higher number of fibres showing focal areas lacking staining for α-actinin, titin and nebulin than in biopsies taken from control or 1 h after exercise. None of these proteins were part of Z-disc streamings but instead they were found in distinct patterns in areas characterised by altered staining for desmin and actin. These were preferentially seen in regions with increased numbers of sarcomeres in parallel myofibrils. We propose that these staining patterns represent different stages of sarcomere formation. These findings therefore support our previous suggestion that muscle fibres subjected to eccentric contractions adapt to unaccustomed activity by the addition of new sarcomeres. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Histochemistry and Cell Biology Springer Journals

The mode of myofibril remodelling in human skeletal muscle affected by DOMS induced by eccentric contractions

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Publisher
Springer Journals
Copyright
Copyright © 2003 by Springer-Verlag
Subject
Legacy
ISSN
0948-6143
eISSN
1432-119X
DOI
10.1007/s00418-003-0522-7
pmid
12712356
Publisher site
See Article on Publisher Site

Abstract

Ji-Guo Yu Dieter O. Fürst Lars-Eric Thornell +46-90-7865142 +46-90-7865480 lars-eric.thornell@anatomy.umu.se Department of Integrative Medical Biology, Section for Anatomy, Umeå University, 901 87, Umeå, Sweden Department of Musculoskeletal Research, Gävle University, 907 13, Gävle, Sweden Department of Cell Biology, Institute for Biochemistry and Biology, University of Potsdam, Potsdam, Germany Myofibrillar Z-disc streaming and loss of the desmin cytoskeleton are considered the morphological hallmarks of eccentric contraction-induced injury. The latter is contradicted by recent studies where a focal increase of desmin was observed in biopsies taken from human muscles with DOMS. In order to determine the effects of eccentric contraction-induced alterations of the myofibrillar Z-disc, we examined the distribution of α-actinin, the Z-disc portion of titin and the nebulin NB2 region in relation to actin and desmin in DOMS biopsies. In biopsies taken 2–3 days and 7–8 days after exercise, we observed a significantly higher number of fibres showing focal areas lacking staining for α-actinin, titin and nebulin than in biopsies taken from control or 1 h after exercise. None of these proteins were part of Z-disc streamings but instead they were found in distinct patterns in areas characterised by altered staining for desmin and actin. These were preferentially seen in regions with increased numbers of sarcomeres in parallel myofibrils. We propose that these staining patterns represent different stages of sarcomere formation. These findings therefore support our previous suggestion that muscle fibres subjected to eccentric contractions adapt to unaccustomed activity by the addition of new sarcomeres.

Journal

Histochemistry and Cell BiologySpringer Journals

Published: May 1, 2003

References

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