The effect of glutathione on the vitamin E requirement for inhibition of liver microsomal lipid peroxidation

The effect of glutathione on the vitamin E requirement for inhibition of liver microsomal lipid... Vitamin E dependent inhibition of rat liver microsomal lipid peroxidation in an NADPH and ADP-Fe +3 containing system occurred at lower vitamin E concentrations in the presence of glutathione (GSH). Using microsomes from rats fed a vitamin E deficient diet, vitamin E was shown to be rquired for inhibition. Inhibition also required the presence of a storage labile microsomal component, since no inhibition was observed when using microsomes that had been stored for one moth. This observation provides evidence that direct reduction of reversibly oxidized vitamin E by GSH does not appear to contribute significantly to inhibition of peroxidation. During GSH and vitamin E dependent inhibition of lipid peroxidation, vitamin E (reduced form) concentrations remained constant, indicating that GSH maintained vitamin E concentrations. Without GSH, vitamin E concentrations dropped rapidly. By adding vitamin E to microsomes, it was found that inhibition of lipid peroxidation in the presence of GSH occurred at about five-fold less vitamin E than in the absence of GSH. Inhibition at these lower levels of vitamin E was 85–90% complete. Results indicate that GSH can be used to maintain vitamin E (reduced form) concentrations, thereby lowering the concentration of vitamin E necessary to inhibit microsomal lipid peroxidation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Lipids Springer Journals

The effect of glutathione on the vitamin E requirement for inhibition of liver microsomal lipid peroxidation

Lipids, Volume 25 (5) – May 1, 1990

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Publisher
Springer Journals
Copyright
Copyright © 1990 by American Oil Chemists’ Society
Subject
Life Sciences; Nutrition; Bioorganic Chemistry; Medicinal Chemistry; Medical Biochemistry; Biochemistry, general; Microbial Genetics and Genomics
ISSN
0024-4201
eISSN
1558-9307
DOI
10.1007/BF02544382
Publisher site
See Article on Publisher Site

Abstract

Vitamin E dependent inhibition of rat liver microsomal lipid peroxidation in an NADPH and ADP-Fe +3 containing system occurred at lower vitamin E concentrations in the presence of glutathione (GSH). Using microsomes from rats fed a vitamin E deficient diet, vitamin E was shown to be rquired for inhibition. Inhibition also required the presence of a storage labile microsomal component, since no inhibition was observed when using microsomes that had been stored for one moth. This observation provides evidence that direct reduction of reversibly oxidized vitamin E by GSH does not appear to contribute significantly to inhibition of peroxidation. During GSH and vitamin E dependent inhibition of lipid peroxidation, vitamin E (reduced form) concentrations remained constant, indicating that GSH maintained vitamin E concentrations. Without GSH, vitamin E concentrations dropped rapidly. By adding vitamin E to microsomes, it was found that inhibition of lipid peroxidation in the presence of GSH occurred at about five-fold less vitamin E than in the absence of GSH. Inhibition at these lower levels of vitamin E was 85–90% complete. Results indicate that GSH can be used to maintain vitamin E (reduced form) concentrations, thereby lowering the concentration of vitamin E necessary to inhibit microsomal lipid peroxidation.

Journal

LipidsSpringer Journals

Published: May 1, 1990

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