Synergism between the chaperone-like activity of the stress regulated ASR1 protein and the osmolyte glycine-betaine

Synergism between the chaperone-like activity of the stress regulated ASR1 protein and the... Abiotic stress may result in protein denaturation. To confront protein inactivation, plants activate protective mechanisms that include chaperones and chaperone-like proteins, and low-molecular weight organic molecules, known as osmolytes or compatible solutes. If these protective processes fail, the irreversibly damaged proteins are targeted for degradation. Tomato ASR1 (SlASR1) is encoded by a plant-specific gene. Steady state levels of transcripts and protein are transiently induced by salt and water stress in an ABA-dependent manner. SlASR1 is localized in both the cytosol as unstructured monomers and in the nucleus as structured DNA-bound dimers. We show here that the unstructured form of SlASR1 has chaperone-like activity and can stabilize a number of proteins against denaturation caused by heat and freeze-thaw cycles. The protective activity of SlASR1 is synergistic with that of the osmolyte glycine-betaine, which accumulates under stress conditions. We suggest that the cytosolic pool of ASR1 protects proteins from denaturation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Planta Springer Journals

Synergism between the chaperone-like activity of the stress regulated ASR1 protein and the osmolyte glycine-betaine

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Publisher
Springer-Verlag
Copyright
Copyright © 2008 by Springer-Verlag
Subject
Life Sciences; Forestry; Ecology; Agriculture; Plant Sciences
ISSN
0032-0935
eISSN
1432-2048
D.O.I.
10.1007/s00425-008-0693-5
Publisher site
See Article on Publisher Site

Abstract

Abiotic stress may result in protein denaturation. To confront protein inactivation, plants activate protective mechanisms that include chaperones and chaperone-like proteins, and low-molecular weight organic molecules, known as osmolytes or compatible solutes. If these protective processes fail, the irreversibly damaged proteins are targeted for degradation. Tomato ASR1 (SlASR1) is encoded by a plant-specific gene. Steady state levels of transcripts and protein are transiently induced by salt and water stress in an ABA-dependent manner. SlASR1 is localized in both the cytosol as unstructured monomers and in the nucleus as structured DNA-bound dimers. We show here that the unstructured form of SlASR1 has chaperone-like activity and can stabilize a number of proteins against denaturation caused by heat and freeze-thaw cycles. The protective activity of SlASR1 is synergistic with that of the osmolyte glycine-betaine, which accumulates under stress conditions. We suggest that the cytosolic pool of ASR1 protects proteins from denaturation.

Journal

PlantaSpringer Journals

Published: Feb 13, 2008

References

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