Sulfated polysaccharides from marine green algae Ulva conglobata and their anticoagulant activity

Sulfated polysaccharides from marine green algae Ulva conglobata and their anticoagulant activity Sulfated polysaccharides from the green algae Ulva conglobata were isolated and prepared by extraction in hot water, precipitation with ethanol and purification by ion-exchange and size-exclusion column chromatography. The characterizations of the sulfated polysaccharides were defined, and containing 23.04–35.20% sulfate ester groups, 10.82–14.91% uronic acid and 3.82–4.51% protein. Gas chromatography analysis shows that the sulfated polysaccharides from Ulva conglobata are mainly consisted of rhamnose with variable contents of glucose and fucose, trace amounts of xylose, glactose and mannose. The anticoagulant properties of the sulfated polysaccharides were compared with those of heparin by studying the activated partial thromboplastin time using normal human plasma. The sulfated polysaccharide from Ulva conglobata collected in Qingdao, China is the most potent among the sulfated polysaccharides tested. The mechanism of anticoagulant activity mediated by the sulfated polysaccharides is due to the direct inhibition of thrombin and the potentiation of heparin cofactor II. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Phycology Springer Journals

Sulfated polysaccharides from marine green algae Ulva conglobata and their anticoagulant activity

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Publisher
Springer Journals
Copyright
Copyright © 2006 by Springer Science+Business Media, Inc.
Subject
Life Sciences; Ecology; Plant Sciences ; Plant Physiology; Freshwater & Marine Ecology
ISSN
0921-8971
eISSN
1573-5176
D.O.I.
10.1007/s10811-005-9008-4
Publisher site
See Article on Publisher Site

Abstract

Sulfated polysaccharides from the green algae Ulva conglobata were isolated and prepared by extraction in hot water, precipitation with ethanol and purification by ion-exchange and size-exclusion column chromatography. The characterizations of the sulfated polysaccharides were defined, and containing 23.04–35.20% sulfate ester groups, 10.82–14.91% uronic acid and 3.82–4.51% protein. Gas chromatography analysis shows that the sulfated polysaccharides from Ulva conglobata are mainly consisted of rhamnose with variable contents of glucose and fucose, trace amounts of xylose, glactose and mannose. The anticoagulant properties of the sulfated polysaccharides were compared with those of heparin by studying the activated partial thromboplastin time using normal human plasma. The sulfated polysaccharide from Ulva conglobata collected in Qingdao, China is the most potent among the sulfated polysaccharides tested. The mechanism of anticoagulant activity mediated by the sulfated polysaccharides is due to the direct inhibition of thrombin and the potentiation of heparin cofactor II.

Journal

Journal of Applied PhycologySpringer Journals

Published: Apr 7, 2006

References

  • Relationship between sulfate groups and biological activities of fucans
    Haroun-Bouhedia, F; Ellouali, M; Sinquin, C; Boisson-Videl, C
  • Heparinoid-active sulfated polysaccharides from
    Maeda, M; Uehara, T; Harada, N; Sekiguchi, M; Hiraoka, A
  • Chemical characterization and radioprotective effect of polysaccharide from Monostroma angicava (Chlorophyta)
    WJ, Mao; Li, Y; Wu, LG; Wang, HQ; Zhang, Y; Zang, XX; Zhang, HJ

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