Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Substrate and light dependent fixation of molecular nitrogen in Rhodospirillum rubrum

Substrate and light dependent fixation of molecular nitrogen in Rhodospirillum rubrum 203 75 75 2 2 H. -J. Schick Institute of Molecular Biophysics Florida State University 32306 Tallahanssee Florida USA Drosselweg 3 D-6232 Bad Soden Summary Whole cells of Rhodospirillum rubrum were cultivated in a malate medium lacking bound nitrogen under N 2 and tested for their nitrogenase activity by measuring the disappearance of nitrogen manometrically. Several experimental conditions were relevant in maintaining consistently high activities. These included: a) light intensity, b) substrate concentration, c) concentration of the cell suspension, d) buffer molarity, pH, and e) temperature. Under our optimal experimental conditions about 6 moles of either l -malate, fumarate, succinate or 10 moles of pyruvate were consumed per 1 mole of molecular nitrogen. The amount of gas taken up by the cells agreed quantitatively with the increase of bound nitrogen found in the cells by microkjeldahl determinations. The fixation of molecular nitrogen is suppresed quickly and specifically by very small amounts of ammonia (<5 μg ammonia N/ml). The duration of this inhibition depends on the amount of ammonia available to the cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

Substrate and light dependent fixation of molecular nitrogen in Rhodospirillum rubrum

Archives of Microbiology , Volume 75 (2) – Jun 1, 1971

Loading next page...
 
/lp/springer-journals/substrate-and-light-dependent-fixation-of-molecular-nitrogen-in-ENJB8WECtP

References (26)

Publisher
Springer Journals
Copyright
Copyright © 1971 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Biochemistry, general; Cell Biology; Ecology; Microbial Ecology; Microbiology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/BF00407997
Publisher site
See Article on Publisher Site

Abstract

203 75 75 2 2 H. -J. Schick Institute of Molecular Biophysics Florida State University 32306 Tallahanssee Florida USA Drosselweg 3 D-6232 Bad Soden Summary Whole cells of Rhodospirillum rubrum were cultivated in a malate medium lacking bound nitrogen under N 2 and tested for their nitrogenase activity by measuring the disappearance of nitrogen manometrically. Several experimental conditions were relevant in maintaining consistently high activities. These included: a) light intensity, b) substrate concentration, c) concentration of the cell suspension, d) buffer molarity, pH, and e) temperature. Under our optimal experimental conditions about 6 moles of either l -malate, fumarate, succinate or 10 moles of pyruvate were consumed per 1 mole of molecular nitrogen. The amount of gas taken up by the cells agreed quantitatively with the increase of bound nitrogen found in the cells by microkjeldahl determinations. The fixation of molecular nitrogen is suppresed quickly and specifically by very small amounts of ammonia (<5 μg ammonia N/ml). The duration of this inhibition depends on the amount of ammonia available to the cells.

Journal

Archives of MicrobiologySpringer Journals

Published: Jun 1, 1971

There are no references for this article.