Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Studies on the physicochemical properties of the new preparation deltaferon as a component of a molecular construct

Studies on the physicochemical properties of the new preparation deltaferon as a component of a... A molecular construct of a spherical shape containing double-stranded RNA (dsRNA) in the center, coated by a spermidine-polyglucine coat, and carrying recombinant deltaferon—an analogue of recombinant interferon-gamma—has been designed. It is shown that deltaferon in this construct retains its specific activity at a level of 9 × 104 U/mg. It was found that deltaferon and dsRNA in the construct were characterized by enhanced resistance to proteases (trypsin) and nucleases (E. coli ribonuclease) as compared to free deltaferon and dsRNA. An increased resistance of deltaferon in the construct to environmental temperature during storage was also demonstrated. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Studies on the physicochemical properties of the new preparation deltaferon as a component of a molecular construct

Download PDF
4 pages

Loading next page...
 
/lp/springer-journals/studies-on-the-physicochemical-properties-of-the-new-preparation-Xau6MpbKAD

References (12)

Publisher
Springer Journals
Copyright
Copyright © 2013 by Pleiades Publishing, Inc.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683813070041
Publisher site
See Article on Publisher Site

Abstract

A molecular construct of a spherical shape containing double-stranded RNA (dsRNA) in the center, coated by a spermidine-polyglucine coat, and carrying recombinant deltaferon—an analogue of recombinant interferon-gamma—has been designed. It is shown that deltaferon in this construct retains its specific activity at a level of 9 × 104 U/mg. It was found that deltaferon and dsRNA in the construct were characterized by enhanced resistance to proteases (trypsin) and nucleases (E. coli ribonuclease) as compared to free deltaferon and dsRNA. An increased resistance of deltaferon in the construct to environmental temperature during storage was also demonstrated.

Journal

Applied Biochemistry and Microbiology – Springer Journals

Published: Oct 22, 2013

There are no references for this article.