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- Publisher
- Springer Journals
- Copyright
- Copyright © Pleiades Publishing, Inc. 2020. ISSN 0003-6838, Applied Biochemistry and Microbiology, 2020, Vol. 56, No. 6, pp. 671–677. © Pleiades Publishing, Inc., 2020. Russian Text © The Author(s), 2020, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2020, Vol. 56, No. 6, pp. 587–594.
- ISSN
- 0003-6838
- eISSN
- 1608-3024
- DOI
- 10.1134/S0003683820060071
- Publisher site
- See Article on Publisher Site
Abstract
CRISPR/Cas13a nucleases are currently considered to be the basis for the development of a new generation of biosensors for the ultrasensitive, in-field detection of bacterial and viral pathogens. A recombinant Cas13a nuclease with functional affinity was obtained as a result of heterologous expression in E. coli with a single-step purification process via metal-chelating chromatography with the N-terminal polyhistidine tag. The simplified procedure of Cas13a nuclease purification broadens the possibilities for the development and practical application of diagnostic biosensing systems based on it. Moreover, our results indicate that the currently uncharacterized protein U2PWF1 of Leptotrichia wadei represents Cas13a nuclease.
Journal
Applied Biochemistry and Microbiology – Springer Journals
Published: Dec 1, 2020