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Simultaneous Determination of Nine Flavonoids in Dalbergia odorifera by LC

Simultaneous Determination of Nine Flavonoids in Dalbergia odorifera by LC A rapid and accurate HPLC method has been developed for the simultaneous determination of nine major flavonoids, namely 3’-hydroxymelanettin, melanettin, stevenin, butein, isoliquiritigenin, dalbergin, 2,4-dihydroxy-5-methoxybenzophenone, pinocembrin, 4-methoxydalbergione in Dalbergia odorifera. The samples were separated on an Aglient Zorbax SB-C18 column (250 × 4.6 mm, 5 μm) with a gradient of acetonitrile and 1% aqueous acetic acid (ν/ν) at a flow rate of 1.0 mL min−1 and detected at 350 nm. The complete separation was obtained within 30 min for the nine target flavonoids. All calibration curves showed good linearity (r2 > 0.999) within test ranges. The repeatability was evaluated by intra- and inter-day assays and RSD values were less than 4.0%. The recoveries were between 92.0% and 104.5%. The developed method was successfully applied to the analysis of 32 commercial samples of D. odorifera. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Chromatographia Springer Journals

Simultaneous Determination of Nine Flavonoids in Dalbergia odorifera by LC

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References (18)

Publisher
Springer Journals
Copyright
Copyright © 2005 by Friedr. Vieweg&Sohn/GWV Fachverlage GmbH
Subject
Chemistry; Chromatography; Proteomics; Pharmacy; Laboratory Medicine
ISSN
0009-5893
eISSN
1612-1112
DOI
10.1365/s10337-005-0520-0
Publisher site
See Article on Publisher Site

Abstract

A rapid and accurate HPLC method has been developed for the simultaneous determination of nine major flavonoids, namely 3’-hydroxymelanettin, melanettin, stevenin, butein, isoliquiritigenin, dalbergin, 2,4-dihydroxy-5-methoxybenzophenone, pinocembrin, 4-methoxydalbergione in Dalbergia odorifera. The samples were separated on an Aglient Zorbax SB-C18 column (250 × 4.6 mm, 5 μm) with a gradient of acetonitrile and 1% aqueous acetic acid (ν/ν) at a flow rate of 1.0 mL min−1 and detected at 350 nm. The complete separation was obtained within 30 min for the nine target flavonoids. All calibration curves showed good linearity (r2 > 0.999) within test ranges. The repeatability was evaluated by intra- and inter-day assays and RSD values were less than 4.0%. The recoveries were between 92.0% and 104.5%. The developed method was successfully applied to the analysis of 32 commercial samples of D. odorifera.

Journal

Chromatographia – Springer Journals

Published: Jan 1, 2005

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