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Selection and characterization of active psychrotrophic microbial oil-degrading microorganisms

Selection and characterization of active psychrotrophic microbial oil-degrading microorganisms The ability of 96 microbial strains degrading oil and 32 strains degrading polycyclic aromatic hydrocarbons (PAHs) to consume diesel fuel and oil at 4–6 and 24°C and at elevated NaCl concentrations was studied. The temperature range, salt tolerance, ability to produce biosurfactants, range of substrates, and antibiotic resistance were determined. The eleven most active oil-degrading and PAH-degrading strains were genotyped by a polymerase chain reaction with BoxA1R primers and a restriction analysis of ribosomal DNA amplicons. For six strains, the degree of oil degradation at 4–6°C was higher than at 24°C. For the most active strains, the degree of oil degradation in liquid mineral medium ranged from 15 to 26% at 24°C and from 28 to 47% at 4–6°C. An association of six of the strains degraded the oil by 46% at 24°C. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Selection and characterization of active psychrotrophic microbial oil-degrading microorganisms

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References (17)

Publisher
Springer Journals
Copyright
Copyright © 2006 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Medical Microbiology; Biochemistry, general; Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683806030070
Publisher site
See Article on Publisher Site

Abstract

The ability of 96 microbial strains degrading oil and 32 strains degrading polycyclic aromatic hydrocarbons (PAHs) to consume diesel fuel and oil at 4–6 and 24°C and at elevated NaCl concentrations was studied. The temperature range, salt tolerance, ability to produce biosurfactants, range of substrates, and antibiotic resistance were determined. The eleven most active oil-degrading and PAH-degrading strains were genotyped by a polymerase chain reaction with BoxA1R primers and a restriction analysis of ribosomal DNA amplicons. For six strains, the degree of oil degradation at 4–6°C was higher than at 24°C. For the most active strains, the degree of oil degradation in liquid mineral medium ranged from 15 to 26% at 24°C and from 28 to 47% at 4–6°C. An association of six of the strains degraded the oil by 46% at 24°C.

Journal

Applied Biochemistry and Microbiology – Springer Journals

Published: May 10, 2006

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