Rice folate enhancement through metabolic engineering has an impact on rice seed metabolism, but does not affect the expression of the endogenous folate biosynthesis genes

Rice folate enhancement through metabolic engineering has an impact on rice seed metabolism, but... Folates are key-players in one-carbon metabolism in all organisms. However, only micro-organisms and plants are able to synthesize folates de novo and humans rely entirely on their diet as a sole folate source. As a consequence, folate deficiency is a global problem. Although different strategies are currently implemented to fight folate deficiency, up until now, all of them have their own drawbacks. As an alternative and complementary means to those classical strategies, folate biofortification of rice by metabolic engineering was successfully achieved a couple of years ago. To gain more insight into folate biosynthesis regulation and the effect of folate enhancement on general rice seed metabolism, a transcriptomic study was conducted in developing transgenic rice seeds, overexpressing 2 genes of the folate biosynthetic pathway. Upon folate enhancement, the expression of 235 genes was significantly altered. Here, we show that rice folate biofortification has an important effect on folate dependent, seed developmental and plant stress response/defense processes, but does not affect the expression of the endogenous folate biosynthesis genes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Rice folate enhancement through metabolic engineering has an impact on rice seed metabolism, but does not affect the expression of the endogenous folate biosynthesis genes

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Publisher
Springer Netherlands
Copyright
Copyright © 2013 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-013-0091-7
Publisher site
See Article on Publisher Site

Abstract

Folates are key-players in one-carbon metabolism in all organisms. However, only micro-organisms and plants are able to synthesize folates de novo and humans rely entirely on their diet as a sole folate source. As a consequence, folate deficiency is a global problem. Although different strategies are currently implemented to fight folate deficiency, up until now, all of them have their own drawbacks. As an alternative and complementary means to those classical strategies, folate biofortification of rice by metabolic engineering was successfully achieved a couple of years ago. To gain more insight into folate biosynthesis regulation and the effect of folate enhancement on general rice seed metabolism, a transcriptomic study was conducted in developing transgenic rice seeds, overexpressing 2 genes of the folate biosynthetic pathway. Upon folate enhancement, the expression of 235 genes was significantly altered. Here, we show that rice folate biofortification has an important effect on folate dependent, seed developmental and plant stress response/defense processes, but does not affect the expression of the endogenous folate biosynthesis genes.

Journal

Plant Molecular BiologySpringer Journals

Published: Jun 16, 2013

References

  • Folate synthesis in plants: the last step of the p-aminobenzoate branch is catalyzed by a plastidial aminodeoxychorismate lyase
    Basset, GJC; Ravanel, S; Quinlivan, EP; White, R; Giovannoni, JJ; Rébeillé, F; Nichols, BP; Shinozaki, K; Seki, M; Gregory, JF; Hanson, AD
  • EXS, a putative LRR receptor kinase, regulates male germline cell number and tapetal identity and promotes seed development in Arabidopsis
    Canales, C; Bhatt, AM; Scott, R; Dickinson, H
  • Interactions between folate and aging for carcinogenesis
    Choi, SW; Friso, S
  • Disruption of histidine catabolism in NEUT2 mice
    Cook, RJ
  • Ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) for the sensitive determination of folates in rice
    Brouwer, V; Storozhenko, S; Stove, CP; Daele, J; Straeten, D; Lambert, WE
  • Arabidopsis haiku mutants reveal new controls of seed size by endosperm
    Garcia, D; Saingery, V; Chambrier, P; Mayer, U; Jurgens, G; Berger, F
  • Maternal control of integument cell elongation and zygotic control of endosperm growth are coordinated to determine seed size in Arabidopsis
    Garcia, D; Fitz Gerald, JN; Berger, F
  • Exploration, normalization, and summaries of high density oligonucleotide array probe level data
    Irizarry, RA; Hobbs, B; Collin, F; Beazer-Barclay, YD; Antonellis, KJ; Scherf, U; Speed, TP
  • The mitochondrial folylpolyglutamate synthetase gene is required for nitrogen utilization during early seedling development in Arabidopsis
    Jiang, L; Liu, Y; Sun, H; Han, Y; Li, J; Li, C; Guo, W; Meng, H; Li, S; Fan, Y; Zhang, C

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