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J Stejskal, M Griga (1992)
Somatic embryogenesis and plant regeneration in Pisum sativum L.Biol. Plant, 34
W. Kysely, H. Jacobsen (2004)
Somatic embryogenesis from pea embryos and shoot apicesPlant Cell, Tissue and Organ Culture, 20
(1979)
In vitro induced morphogenesis in pea embryos
O. Gamborg, R. Miller, K. Ojima (1968)
Nutrient requirements of suspension cultures of soybean root cells.Experimental cell research, 50 1
T. Tétu, R. Sangwan, B. Sangwan-Norreel (1990)
Direct somatic embryogenesis and organogenesis in cultured immature zygotic embryos of Pisum sativum L.Journal of Plant Physiology, 137
(1992)
Biotechnology applied to grain legumes - current state and approaches
SA Plohinski (1970)
Biometrics
H. Kallak, A. Kǒiveer (1990)
Induction of morphogenesis in meristems of different cultivars of Pisum sativum L.Plant Science, 67
S. Özcan, M. Barghchi, S. Firek, J. Draper (1992)
High frequency adventitious shoot regeneration from immature cotyledons of pea (Pisum sativum L.)Plant Cell Reports, 11
L. Doorne, G. Marshall, R. Kirkwood (1995)
Somatic embryogenesis in pea (Pisum sativum L): Effect of explant, genotype and culture conditionsAnnals of Applied Biology, 126
Ten genotypes from Pisum sativum and Pisum arvense were screened for their regeneration abilities. Most of them were created through experimental mutagenesis from Bulgarian varieties and have various valuable agronomic traits. Embryonic axes from immature embryos were plated on modified Murashige and Skoog medium, containing different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-d), α-naphthaleneacetic acid (NAA) and benzyladenine (BA). Two schemes for direct and indirect organogenesis were established. Callus and shoot formation were induced on media containing 0.2 mM 2,4-d or 5 mM BA, respectively. Embryonic axes formed buds directly when plated on medium with 10 mM BA and 1 mM NAA. Organogenesis and adventitious bud formation were maintained on medium supplemented with BA and NAA. Rhizogenesis was induced on Gamborgs' B5 medium. All screened genotypes were able to regenerate plants with a high efficiency (50–100%) although some differences in their organogenetic response were observed.
Plant Cell, Tissue and Organ Culture – Springer Journals
Published: Sep 29, 2004
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