Rapid separation of neutral lipids, free fatty acids and polar lipids using prepacked silica sep-Pak columns

Rapid separation of neutral lipids, free fatty acids and polar lipids using prepacked silica... A method is described for the separation of neutral lipid, free fatty acid and polar lipid classes using small (600 mg), prepacked silica Sep-Pak columns. Combinations of hexane and methyltertiarybutylether were used to progressively elute cholesteryl ester first then triglyceride from the column. After column acidification, fatty acids were eluted followed by cholesterol. Recoveries of these lipids were 96% or greater. Polar lipids were eluted from the column using combinations of methyltertiarybutylether, methanol and ammonium acetate. Phospholipid classes could not be separated completely from each other. Phosphatidylethanolamine and phosphatidylinositol eluted together, whereas the more polar phosphatidylcholine, sphingomyelin and lysophosphatidylcholine were eluted as a second fraction. Recoveries of each phospholipid was greater than 98%. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Lipids Springer Journals

Rapid separation of neutral lipids, free fatty acids and polar lipids using prepacked silica sep-Pak columns

Lipids, Volume 23 (12) – Dec 1, 1988

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Publisher
Springer Journals
Copyright
Copyright © 1988 by American Oil Chemists’ Society
Subject
Life Sciences; Nutrition; Bioorganic Chemistry; Medicinal Chemistry; Medical Biochemistry; Biochemistry, general; Microbial Genetics and Genomics
ISSN
0024-4201
eISSN
1558-9307
DOI
10.1007/BF02535281
Publisher site
See Article on Publisher Site

Abstract

A method is described for the separation of neutral lipid, free fatty acid and polar lipid classes using small (600 mg), prepacked silica Sep-Pak columns. Combinations of hexane and methyltertiarybutylether were used to progressively elute cholesteryl ester first then triglyceride from the column. After column acidification, fatty acids were eluted followed by cholesterol. Recoveries of these lipids were 96% or greater. Polar lipids were eluted from the column using combinations of methyltertiarybutylether, methanol and ammonium acetate. Phospholipid classes could not be separated completely from each other. Phosphatidylethanolamine and phosphatidylinositol eluted together, whereas the more polar phosphatidylcholine, sphingomyelin and lysophosphatidylcholine were eluted as a second fraction. Recoveries of each phospholipid was greater than 98%.

Journal

LipidsSpringer Journals

Published: Dec 1, 1988

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