Quantitative Evidence for Persistence of Human Parvovirus B19 DNA in an Immunocompetent Individual

Quantitative Evidence for Persistence of Human Parvovirus B19 DNA in an Immunocompetent Individual Eur J Clin Microbiol Infect Dis (2000) 19 : 886–895 Q Springer-Verlag 2000 amplification (1 cycle at 50 7C for 2 min, 95 7C for Brief Reports 10 min, 45 cycles at 95 7C for 15 s and 60 7C for 1 min) in an ABI Prism 7700 Sequence Detection System Quantitative Evidence for (Applied Biosystems, USA) using optical tubes and caps. An acute parvovirus B19 infection was serologi- Persistence of Human Parvovirus cally documented by the demonstration of anti-B19 B19 DNA in an Immunocompetent IgM antibody, but no anti-B19 IgG antibody, in the first blood sample collected at presentation. Up to Individual 8.8!10 ge/ml was measured in this blood sample. A cerebrospinal fluid sample collected at the same time P. Cassinotti, G. Siegl contained 2.6!10 ge/ml. The viral load dropped to 6 3 2.2!10 ge/ml and 6!10 ge/ml in blood samples Human parvovirus B19 infections are generally acute collected 1 and 11 weeks later, respectively. The latter and self-limiting. However, by means of qualitative sample contained anti-B19 IgG antibody, but IgM anti- polymerase chain reaction (PCR) analysis, persistent body was no longer present. infections have been demonstrated in immunocompe- tent individuals by detection of parvovirus B19 DNA in http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Clinical Microbiology Infectious Diseases Springer Journals

Quantitative Evidence for Persistence of Human Parvovirus B19 DNA in an Immunocompetent Individual

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Publisher
Springer Journals
Copyright
Copyright © 2000 by Springer-Verlag Berlin Heidelberg
Subject
Biomedicine; Medical Microbiology; Internal Medicine
ISSN
0934-9723
eISSN
1435-4373
DOI
10.1007/s100960000384
Publisher site
See Article on Publisher Site

Abstract

Eur J Clin Microbiol Infect Dis (2000) 19 : 886–895 Q Springer-Verlag 2000 amplification (1 cycle at 50 7C for 2 min, 95 7C for Brief Reports 10 min, 45 cycles at 95 7C for 15 s and 60 7C for 1 min) in an ABI Prism 7700 Sequence Detection System Quantitative Evidence for (Applied Biosystems, USA) using optical tubes and caps. An acute parvovirus B19 infection was serologi- Persistence of Human Parvovirus cally documented by the demonstration of anti-B19 B19 DNA in an Immunocompetent IgM antibody, but no anti-B19 IgG antibody, in the first blood sample collected at presentation. Up to Individual 8.8!10 ge/ml was measured in this blood sample. A cerebrospinal fluid sample collected at the same time P. Cassinotti, G. Siegl contained 2.6!10 ge/ml. The viral load dropped to 6 3 2.2!10 ge/ml and 6!10 ge/ml in blood samples Human parvovirus B19 infections are generally acute collected 1 and 11 weeks later, respectively. The latter and self-limiting. However, by means of qualitative sample contained anti-B19 IgG antibody, but IgM anti- polymerase chain reaction (PCR) analysis, persistent body was no longer present. infections have been demonstrated in immunocompe- tent individuals by detection of parvovirus B19 DNA in

Journal

European Journal of Clinical Microbiology Infectious DiseasesSpringer Journals

Published: Dec 5, 2000

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