Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Quantitative Determination of Acyclovir in Aqueous Humor by LC-MS

Quantitative Determination of Acyclovir in Aqueous Humor by LC-MS A specific, sensitive and precise liquid chromatographic assay method was established using LC-MS for the determination of acyclovir (ACV) in aqueous humor (AH), which was directly injected onto an Inertsil ODS-3 C18 column without any pretreatment. The Agilent 1100 series LC–MS system was operated under the electrospray ionization mode (ESI). The analyte was separated from endogenous substances with a mobile phase of methanol: water: acetic acid (5:95:0.1, v/v) at a flow-rate of 0.3mL min−1. A linear response was observed over the concentration range from 5 to 50ng mL−1 (r=0.9993). Intra- and inter-day coefficients of variation were in the ranges 5.2–9.0% and 5.8–8.2%, respectively. The recovery was > 91.0%, and the limit of detection was approximate 1ng mL−1. The pharmacokinetics of topically applied eye-drop and thermosetting gel were compared in rabbits utilizing the present method, the results demonstrated that LC-MS was a powerful tool for the detection of ACV in AH. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Chromatographia Springer Journals

Quantitative Determination of Acyclovir in Aqueous Humor by LC-MS

Jin, Liang; Wei, Gang; Lu, Wei-Yue; Xu, Ling-Jie; Pan, Jun
Chromatographia , Volume 63 (6) – Feb 28, 2006
Download PDF
4 pages

Loading next page...
 
/lp/springer-journals/quantitative-determination-of-acyclovir-in-aqueous-humor-by-lc-ms-0nLMg4jvb3

References (16)

Publisher
Springer Journals
Copyright
Copyright © 2006 by Friedr. Vieweg & Sohn/GWV Fachverlage GmbH
Subject
Chemistry; Chromatography; Proteomics; Pharmacy; Laboratory Medicine
ISSN
0009-5893
eISSN
1612-1112
DOI
10.1365/s10337-006-0732-y
Publisher site
See Article on Publisher Site

Abstract

A specific, sensitive and precise liquid chromatographic assay method was established using LC-MS for the determination of acyclovir (ACV) in aqueous humor (AH), which was directly injected onto an Inertsil ODS-3 C18 column without any pretreatment. The Agilent 1100 series LC–MS system was operated under the electrospray ionization mode (ESI). The analyte was separated from endogenous substances with a mobile phase of methanol: water: acetic acid (5:95:0.1, v/v) at a flow-rate of 0.3mL min−1. A linear response was observed over the concentration range from 5 to 50ng mL−1 (r=0.9993). Intra- and inter-day coefficients of variation were in the ranges 5.2–9.0% and 5.8–8.2%, respectively. The recovery was > 91.0%, and the limit of detection was approximate 1ng mL−1. The pharmacokinetics of topically applied eye-drop and thermosetting gel were compared in rabbits utilizing the present method, the results demonstrated that LC-MS was a powerful tool for the detection of ACV in AH.

Journal

Chromatographia – Springer Journals

Published: Feb 28, 2006

There are no references for this article.