Buckwheat is a functional staple food, which is rich in rutin and other flavonoids with strong antioxidant potential. However, the quality of buckwheat grain is easy to degrade during storage and low temperature is better to keep the flavor and texture. We tested the hypothesis that the quality degradation is related to seed dormancy which is lost at a rate in temperature-dependent manner in most seeds. To understand physiological processes in seeds under temperature stress, which affects quality of stored buckwheat grain, a gel-free/label-free proteomic analysis was conducted. Compared to the seeds before storage, a total of 30, 76, 52, 14, and 61 proteins were affected at 5 °C for 5 months, 5 °C for 10 months, 15 °C for 5 months, 15 °C for 10 months, and 25 °C for 5 months, respectively. Functional classification revealed that storage condition at 5 °C for 5 months resulted in different protein profile from other conditions and suitable for keeping best condition. Storage at higher temperature for longer time influenced proteins related to preparation for emergence by carbohydrate metabolism, endogenous gene expression, and protein homeostasis. Proteins such as granule-bound starch synthase and 13S seed storage protein consistently decreased in abundance during storage. These results suggest that stored buckwheat seeds experience seed dormancy release, but the processes are not clear in relation to temperature rise. Dormancy break is recognized but emergence is inhibited by abscisic acid, which was induced by temperature stress, showing the complexity of buckwheat germination control. The quality degradation of stored seeds might be due to changed starch composition and protein network.
Theoretical and Experimental Plant Physiology – Springer Journals
Published: May 24, 2018
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera