Properties of plasma membrane H + -ATPase in salt-treated Populus euphratica callus

Properties of plasma membrane H + -ATPase in salt-treated Populus euphratica callus The plasma membrane (PM) vesicles from Populus euphratica ( P. euphratica ) callus were isolated to investigate the properties of the PM H + -ATPase. An enrichment of sealed and oriented right-side-out PM vesicles was demonstrated by measurement of the purity and orientation of membrane vesicles in the upper phase fraction. Analysis of pH optimum, temperature effects and kinetic properties showed that the properties of the PM H + -ATPase from woody plant P. euphratica callus were consistent with those from herbaceous species. Application of various thiol reagents to the reaction revealed that reduced thiol groups were essential to maintain the PM H + -ATPase activity. In addition, there was increased H + -ATPase activity in the PM vesicles when callus was exposed to NaCl. Western blotting analysis demonstrated an enhancement of H + -ATPase content in NaCl-treated P. euphratica callus compared with the control. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

Properties of plasma membrane H + -ATPase in salt-treated Populus euphratica callus

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Publisher
Springer Journals
Copyright
Copyright © 2007 by Springer-Verlag
Subject
Life Sciences; Plant Biochemistry; Biotechnology; Plant Sciences ; Cell Biology
ISSN
0721-7714
eISSN
1432-203X
D.O.I.
10.1007/s00299-006-0220-8
Publisher site
See Article on Publisher Site

Abstract

The plasma membrane (PM) vesicles from Populus euphratica ( P. euphratica ) callus were isolated to investigate the properties of the PM H + -ATPase. An enrichment of sealed and oriented right-side-out PM vesicles was demonstrated by measurement of the purity and orientation of membrane vesicles in the upper phase fraction. Analysis of pH optimum, temperature effects and kinetic properties showed that the properties of the PM H + -ATPase from woody plant P. euphratica callus were consistent with those from herbaceous species. Application of various thiol reagents to the reaction revealed that reduced thiol groups were essential to maintain the PM H + -ATPase activity. In addition, there was increased H + -ATPase activity in the PM vesicles when callus was exposed to NaCl. Western blotting analysis demonstrated an enhancement of H + -ATPase content in NaCl-treated P. euphratica callus compared with the control.

Journal

Plant Cell ReportsSpringer Journals

Published: Feb 1, 2007

References

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