Prokaryotic expression of antibodies

Prokaryotic expression of antibodies Maximizing the expression yields of recombinant whole antibodies and antibody fragments such as Fabs, single-chain Fvs and single-domain antibodies is highly desirable since it leads to lower production costs. Various eukaryotic and prokaryotic expression systems have been exploited to accommodate antibody expression but Escherichia coli systems have enjoyed popularity, in particular with respect to antibody fragments, because of their low cost and convenience. In many instances, product yields have been less than adequate and intrinsic and extrinsic variables have been investigated in an effort to improve yields. This review deals with various aspects of antibody expression in E. coli with a particular focus on single-domain antibodies. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Cancer and Metastasis Reviews Springer Journals

Prokaryotic expression of antibodies

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Publisher
Springer Journals
Copyright
Copyright © 2005 by Springer Science + Business Media, Inc.
Subject
Medicine & Public Health; Cancer Research; Oncology
ISSN
0167-7659
eISSN
1573-7233
D.O.I.
10.1007/s10555-005-6193-1
Publisher site
See Article on Publisher Site

Abstract

Maximizing the expression yields of recombinant whole antibodies and antibody fragments such as Fabs, single-chain Fvs and single-domain antibodies is highly desirable since it leads to lower production costs. Various eukaryotic and prokaryotic expression systems have been exploited to accommodate antibody expression but Escherichia coli systems have enjoyed popularity, in particular with respect to antibody fragments, because of their low cost and convenience. In many instances, product yields have been less than adequate and intrinsic and extrinsic variables have been investigated in an effort to improve yields. This review deals with various aspects of antibody expression in E. coli with a particular focus on single-domain antibodies.

Journal

Cancer and Metastasis ReviewsSpringer Journals

Published: Jan 1, 2005

References

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    Kipriyanov, SM; Gall, F
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    Andersen, DC; Krummen, L
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    Trinh, R; Gurbaxani, B; Morrison, SL; Seyfzadeh, M
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    Corisdeo, S; Wang, B
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    Bardwell, JC
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    Hudson, PJ; Kortt, AA
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    Lauwereys, M; Arbabi-Ghahroud, M; Desmyter, A; Kinne, J; Holzer, W; Gens, E; Wyn, L; Muyldermans, S
  • PEGylated antibodies and antibody fragments for improved therapy: A review
    Chapman, AP

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